GAL4は,哺乳類の細胞における遺伝子発現を活性化する
1Department of Biochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138.
Cell
|January 29, 1988
まとめ
イーストの転写因子GAL4は,哺乳類の細胞における遺伝子発現を活性化させます. その活性化領域とDNA結合能力は,この種間機能にとって極めて重要です.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- イースト遺伝学 イースト遺伝学
背景:
- GAL4は酵母における特異性の高い転写活性化剤である.
- マウス乳腺腫瘍ウイルス (MMTV) プロモーターは,遺伝子発現研究における一般的なリポーターです.
- 異種間の転写因子機能を理解することは,保存された規制メカニズムについての洞察を提供します.
研究 の 目的:
- イーストの転写因子GAL4が哺乳類の細胞での転写を活性化できるかどうかを調査する.
- 哺乳類のシステムで活性化するために必要なGAL4の機能領域を決定する.
- GAL4とグルココルチコイド受容体のMMTVプロモーターに対する相乗効果を調べる.
主な方法:
- 哺乳類の細胞を,GAL4DNA結合配列とレポーター遺伝子を含むプラズミドで感染させる.
- レポーター遺伝子発現の分析.
- GAL4消去変異体とDNA結合欠陥変異体の使用.
- グルココルチコイド受容体発現ベクトルとの共伝染.
主要な成果:
- GAL4は,哺乳類の細胞におけるMMTVプロモーターを活性化する.
- アクティベーションには,プロモーターの上流にあるGAL4結合配列が必要です.
- GAL4の特定の活性化領域は,哺乳類の細胞で機能するために保存されています.
- 酵母では活性化できないがDNAに結合するGAL4誘導体は,哺乳類の細胞でも活性化できない.
- GAL4とグルココルチコイド受容体は,MMTVプロモーターの協同活性化を示す.
結論:
- GAL4転写因子は哺乳類の細胞で機能することができ,保存された規制原理を示しています.
- GAL4内の特定のドメインは,その種間トランスクリプションの活性化に不可欠です.
- GAL4とグルココルチコイド受容体は,遺伝子発現を強化するために協力することができます.
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