完全に組み立てられた状態で凍結したシアノバクテリアの昼間振動器の構造
Joost Snijder1, Jan M Schuller2, Anika Wiegard3
1Biomolecular Mass Spectrometry and Proteomics and Netherlands Proteomics Center, Bijvoet Center for Biomolecular Research, and Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Padualaan 8, 3584 CH, Utrecht, Netherlands.
Science (New York, N.Y.)
|March 18, 2017
まとめ
サイアノバクテリアのカイシステムは,サイアノバクテリアのカイC,カイB,カイAタンパク質を使って再構成された. 先進的な顕微鏡と質量スペクトロメトリーを用いて 24 時間周期の構造的基礎を明らかにしました
科学分野:
- 生物化学
- 構造生物学
- クロノバイオロジー
背景:
- サイアノバクテリアは 健やかな昼夜振動器 (カイシステム) を持っていて 試験管内で再構成できます
- Kaiシステムは,KaiA,KaiB,およびKaiCタンパク質で構成され,アデノシン5'-トリフォスファート (ATP) と独立して機能する.
- KaiCヘクサマーは24時間のオートフォスフォリレーションサイクルを経て,KaiAとKaiBと複合する.
研究 の 目的:
- カイCBとカイCBA複合体のステキオメトリックに定義された集合を準備する.
- クリオ電子顕微鏡と質量スペクトロメトリを用いてこれらのタンパク質を構造的に特徴づける.
- カイの日常振動器内の分子相互作用を解明するために
主な方法:
- タンパク質複合体ステキオメトリーをモニターするためのネイティブ質量スペクトロメトリー.
- 単粒子冷凍電子顕微鏡で構造を決定する.
- カイ循環器の構成要素の生化学的復元
主要な成果:
- 明確に定義された KaiCB と KaiCBA ステキオメトリックアセンブリの準備
- KaiA,KaiB,およびKaiCタンパク質の相互作用に関する構造的洞察.
- カイの日経振動器の組立ダイナミクスの詳細な理解
結論:
- この研究は,サイアノバクテリアの昼夜振動器の周期的組み立てのための構造的基礎を提供します.
- カイシステムの構造を理解することは 昼夜リズムメカニズムの解読に不可欠です
- この研究は 生物学的時計の構造生物学を 進歩させています
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