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関連する概念動画

Replication in Eukaryotes02:31

Replication in Eukaryotes

Overview
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...
The DNA Replication Fork01:02

The DNA Replication Fork

An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication forks, one in...
Replication in Eukaryotes01:29

Replication in Eukaryotes

In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart, a...

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関連する実験動画

Updated: Jul 17, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

イニシエーションタンパク質が誘発した,ラムダ起源のヘリックス不安定化:DNA複製の準備段階である.

M Schnos1, K Zahn, R B Inman

  • 1Institute for Molecular Virology, University of Wisconsin-Madison 53706.

Cell
|February 12, 1988
PubMed
まとめ

ラムダファグのイニシアタータンパク質Oは,起源配列に結合し,複製の開始に不可欠なDNA構造の変化を引き起こします. このプロセスは,DNAの超螺旋的な緊張に依存しており,双方向複製の第一歩です.

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • ラムダファグのイニシアタータンパク質Oは,複製のラムダ起源 (ori) に結合する.
  • DNA複製の初期分子イベントを理解することは,ウイルス伝播とゲノム安定性を理解するために重要です.

研究 の 目的:

  • イニシアタータンパク質O.の結合時のラムダ起源配列の構造変化を調査する.
  • O-ori相互作用におけるDNA超螺旋張の役割とその複製開始への影響を決定する.

主な方法:

  • 核酵素感受性アッセイは,DNAの構造変化を検出するために使用されました.
  • 実験では,観察された変化の機能的関連性を評価するために,野生型と変異型オリの配列を対象とした.

主要な成果:

  • タンパク質Oの結合は,オリ配列の特定のAT豊富な領域における重要な構造変化を誘導する.
  • このDNAの改変はATPの水解とは無関係であり,複製しないオリ変異体によって減少する.
  • 構造の変化は,DNAの超螺旋的な緊張に決定的に依存し,鎖分離の特徴を示します.

結論:

さらに関連する動画

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

関連する実験動画

Last Updated: Jul 17, 2026

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
17:03

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay

Published on: March 23, 2010

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase
07:37

Single-Molecule Real-Time Visualization of DNA Unwinding by CMG Helicase

Published on: September 27, 2024

  • ラムダファグイニシアタータンパク質Oとオリ配列の相互作用により,張力依存のDNA構造変化が誘発されます.
  • この改変は,基本的なプリプリングイベントとして提案されており,ラムダファグにおける双方向DNA複製の開始における最初のステップを表しています.