関連する実験動画
Updated: May 11, 2026

09:40
Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Published on: June 27, 2017
核因子1の結合部位は,成長因子βを変換することによって,I型コラーゲンプロモーターの転写活性化を媒介する
P Rossi1, G Karsenty, A B Roberts
1Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Cell
|February 12, 1988
まとめ
トランスフォーミング成長因子β (TGFβ) はコラーゲン遺伝子転写を活性化します. このプロセスは,特定の核因子1 (NF1) 結合部位を必要とし,TGF-βにおけるその重要な役割を果たしていることを示しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- バイオケミストリー バイオケミストリー
背景:
- 変形成長因子-β (TGF-β) は,線維芽細胞における細胞外マトリックスタンパク質の産生を調節することが知られている.
- TGF-βの作用の根底にある転写メカニズムを理解することは,組織再構成と線維症の研究に不可欠です.
研究 の 目的:
- マウスアルファ2 (I) コラーゲン遺伝子のTGF-β誘発転写活性化を媒介する特定のDNA要素の役割を調査する.
- TGF-βの信号伝達経路に関与する転写因子を特定する.
主な方法:
- NIH 3T3およびオステオサルコマ細胞におけるDNAトランスフェクションアッセイを活用した.
- マウスのα2 (I) コラーゲンプロモーターでデレーションとサイト・ディレクテッド・ミュータゲネシスの分析を行った.
- レポーター遺伝子発現を測定することによってプロモーターの活性度を評価.
- ヘテロログスプロモーター (SV40早期プロモーター) を使用して,特定された規制要素の機能をテストしました.
主要な成果:
- TGF-βはマウスのα2 (I) コラーゲンプロモーターの活性 (5〜10倍) を有意に増加させた.
- 核因子1 (NF1) の結合部位を含む,−350〜−300の間の重要なプロモーター領域は,TGF-β刺激に不可欠でした.
- NF1結合部位の変異により,TGF-β誘発活性化が廃止されました.
- NF1結合部位は,異質プロモーターにTGF-β誘導性を授与し,メディエーターとしての役割を確認した.
結論:
- 核因子1 (NF1) の結合部位は,TGF-βによってマウスのα2 (I) コラーゲンプロモーターの転写活性化を媒介する.
- この発見は,TGF-βが細胞外マトリックス遺伝子発現を調節する重要な分子機構を明らかにしています.
関連する概念動画
Eukaryotic Transcription Activators
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Elongation Factors
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA into a...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Fibril-associated Collagen
Fibril-associated collagens are a type of collagens present in the extracellular matrix with interrupted triple helices or FACIT (Fibril-associated collagens interrupted triple-helices). FACIT help connect and attach the collagen fibrils with each other as well as with other proteins of the extracellular matrix.
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
For example, the type II collagen fibrils in cartilage have covalently bound type IX fibril-associated collagens at regular intervals. Other types of fibril-associated collagens are...
Type IV Collagen of Basal Lamina
Type IV collagen is a 400 nm long, network-forming collagen that acts as a barrier between the epithelial and endothelial cells. Type IV collagen forms the backbone of the basement membrane by scaffolding with laminin, entactin, proteoglycans, and fibronectin. Apart from rendering structural support to the basement membrane, it also helps entail signaling potentials necessary for both pathological and physiological functions.
A type IV collagen molecule has six alpha chains which can exist in...
A type IV collagen molecule has six alpha chains which can exist in...

