細胞成分とHSV構造ポリペプチドの間に形成される複合体は,ウイルスのすぐれた早期遺伝子調節DNA配列に結合します
C M Preston1, M C Frame, M E Campbell
1Medical Research Council Virology Unit, Institute of Virology, Glasgow, Scotland.
Cell
|February 12, 1988
まとめ
ヘルペス・シンプレックスウイルス (HSV) のタンパク質Vmw65は,TAATGARAT配列に結合し,ウイルス誘発タンパク質-DNA複合体 (IEC) を形成します. この複合体にはVmw65と細胞因子が含まれ,遺伝子の転写を調節するのに役立ちます.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 遺伝子規制 遺伝子規制
背景:
- ヘルペス・シンプレックスウイルス (HSV) の早期の遺伝子転写は,ウイルス要因によって制御されます.
- ウイルスのタンパク質Vmw65は,この転写プロセスを刺激することが知られている.
- 特定のDNA配列は,この相互作用に不可欠です.
研究 の 目的:
- Vmw65がHSVの早期遺伝子転写を刺激するメカニズムを調査する.
- この過程で形成されるタンパク質-DNA複合体を特定し,特徴づけること.
- 配列特異のDNA結合におけるVmw65と細胞因子の役割を理解する.
主な方法:
- HSVに感染した細胞の核抽出物を,TAATGARATのDNA断片でインキュベーションする.
- 感染していない細胞からの核抽出物にVmw65を含むビリオン抽出物を添加する.
- ウイルスに誘発されたタンパク質-DNA複合体 (IEC) の検出と特徴付け.
主要な成果:
- 新型ウイルス誘発型タンパク質-DNA複合体 (IEC) が,TAATGARATのDNA断片による核抽出物の化時に検出されました.
- IECの形成は,Vmw65を含むビリオンの抽出物が,感染していない細胞からの核抽出物に加えられたときにも観察されました.
- Vmw65は,特に TAATGARAT 配列に結合する IEC のコンポーネントとして特定されました.
結論:
- Vmw65は,細胞因子と結合して,配列特異のDNA結合複合体 (IEC) を形成する.
- この複合体は,HSVのすぐ前の初期の遺伝子の転写的調節に重要な役割を果たします.
- 特定されたシステムは,誘導可能な細胞遺伝子の調節を研究するためのモデルとして機能します.
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