v-junは,AP-1の強化剤結合特性を持つ核タンパク質をコードする

T J Bos¹, D Bohmann, H Tsuchie

¹Department of Microbiology, University of Southern California, School of Medicine, Los Angeles 90033.

Cell

|March 11, 1988

まとめ

ASV17ウイルスです.

科学分野:

腫瘍性ウイルスの遺伝子発現と機能.
転写因子の分子生物学.
細胞の変容と腫瘍創生.

背景:

鳥肉腫ウイルス17 (ASV17) は,腫瘍遺伝子を宿している.
v-junタンパク質 (p65gag-jun) は,ガグ配列を有する融合タンパク質である.
V-junの生化学的性質を理解することは,それを細胞のc-junと比較するために不可欠です.

研究の目的:

ASV17のv-junタンパク質 (p65gag-jun) の生化学的性質を特徴づけるために.
ウイルスのv-junと細胞のc-junの間の潜在的な機能的違いを評価する.
p65gag-jun.jun.のDNA結合特異性を調査するために.

主な方法:

免疫光顕微鏡で,タンパク質の局所を決定する.
DNAase Iの足跡分析は,DNA結合を評価するために行われます.
結合特異性を確認するために点変異体の分析.

主要な成果:

v-junタンパク質 (p65gag-jun) は,鶏の胚性線維芽細胞 (CEF) の核に局限する.
p65gag-junは,AP-1によって認識されるSV40DNAの強化元に結合する.
v-junタンパク質は,AP-1やGCN4と類似したDNA配列特異結合を示す.

結論:

ウイルスジュン腫瘍遺伝子の産物 (v-jun) は,細胞の転写因子に似たDNA結合特性を有する.
結合腫瘍遺伝子の活性化には,変化したDNA結合以外のメカニズムが含まれている可能性があります.
v-jun.の完全な腫瘍発生可能性を明らかにするために,さらなる研究が必要である.

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