多重で機能的に異なるTATA要素の定義,そのうちの1つは,E1A規制のためのhsp70プロモーターのターゲットである
M C Simon1, T M Fisch, B J Benecke
1Laboratory of Molecular Cell Biology, Howard Hughes Medical Institute Rockefeller University, New York, New York 10021.
Cell
|March 11, 1988
まとめ
人間のhsp70プロモーターは
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオケミストリー バイオケミストリー
背景:
- 人間の熱ショックタンパク質70 (hsp70) プロモーターは,細胞のストレス反応の重要な調節因子です.
- プロモーター要素を理解することは,遺伝子発現の制御に不可欠です.
研究 の 目的:
- E1A媒介誘導を担うhsp70プロモーター内の特定のDNA配列要素を特定する.
- プロモーターの調節と誘導性におけるTATAボックス配列の役割を調査する.
主な方法:
- Bal 31核酵素を用いたデレーション変異は,上流の配列を変更する.
- TATAボックスの領域を特異的に変異させるためのサイト指向型変異.
- クロランフェニコルアセチルトランスフェラーゼ (CAT) を使用したレポーター遺伝子アッセイは,プロモーターの活性を測定します.
主要な成果:
- hsp70 TATA要素のアップストリーム配列は,E1A誘導性に最小の影響を及ぼしました.
- 特定のTATA配列TATAAは,hsp70プロモーターのE1A媒介刺激に不可欠でした.
- TATAA配列をSV40 TATA要素に置き換えることで,熱誘導性を維持しながら,E1A誘導性を廃止しました.
結論:
- TATAA配列は,hsp70プロモーターのE1A誘導の重要な標的である.
- 異なるTATA要素とその関連する転写因子は,真核細胞における微分プロモーターの調節を媒介する.
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