関連する実験動画

Updated: Mar 5, 2026

High-throughput Purification of Affinity-tagged Recombinant Proteins
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High-throughput Purification of Affinity-tagged Recombinant Proteins

Published on: August 26, 2012

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RNAポリメラーゼIトランスクリプションの構造的基礎

Christoph Engel1, Tobias Gubbey1, Simon Neyer1

  • 1Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Cell
|March 25, 2017
PubMed
まとめ

RNAポリメラーゼI (Pol I) と初期因子Rrn3とコア因子 (CF) は,リボソームRNA遺伝子転写に不可欠である. 構造研究は,CFがPol I-Rrn3にドッキングし,DNAをロードし,転写を開始する方法を明らかにします.

キーワード:
RNAポリメラーゼIX線結晶学コア・ファクタークリオ・エムリボソームRNAプロモータートランスクリプション開始

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関連する概念動画

Transcription Initiation01:47

Transcription Initiation

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Bacterial Transcription

RNA polymerase (RNAP) carries out DNA-dependent RNA synthesis in both bacteria and eukaryotes. Bacteria do not have a membrane-bound nucleus. So, transcription and translation occur simultaneously, on the same DNA template.
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General Transcription Factors

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In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
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