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関連する概念動画

Regulated mRNA Transport02:22

Regulated mRNA Transport

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In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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Regulated mRNA Transport

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Nuclear Protein Sorting01:34

Nuclear Protein Sorting

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

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Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
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Nuclear Export of mRNA02:31

Nuclear Export of mRNA

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Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

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スナップショット:サブセルラーmRNAの局所化

Mohammad Mofatteh1, Simon L Bullock1

  • 1Cell Biology Division, MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.

Cell
|March 25, 2017
PubMed
まとめ
この要約は機械生成です。

細胞はメッセンジャーRNA (mRNA) を特定の細胞質部位に定着させ,タンパク質の機能を正確に制御する. このレビューでは,細胞骨格モーターに焦点を当てて,mRNAトラフィックのメカニズムを探索します.

さらに関連する動画

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

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関連する実験動画

Last Updated: Mar 5, 2026

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Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells
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Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation
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Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

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科学分野:

  • 細胞生物学
  • 分子生物学
  • 生物化学

背景:

  • 細胞内のタンパク質合成の空間的および時間的制御には,サブセルラーmRNAの局所化が不可欠である.
  • この過程で 局所的なタンパク質の機能が 可能になり 細胞の様々なプロセスに 影響を及ぼします

研究 の 目的:

  • サブセルラーmRNAの局所化に関与する主要なイベントの概要を説明する.
  • mRNAの密輸における細胞骨格モーターの役割を理解する最近の進歩を強調する.

主な方法:

  • mRNAの局所化と輸送に関する既存の文献のレビュー.
  • 細胞骨格モーターがmRNAと相互作用し,移動するメカニズムの分析.

主要な成果:

  • mRNAの局所化は 精密なタンパク質の生産を可能にする 基本的な細胞プロセスです
  • キネシンやダイネインなどの細胞骨格運動タンパク質は,細胞内のmRNA輸送をオーケストラする上で重要な役割を果たします.

結論:

  • 細胞の機能と発達を理解するには,mRNAの密輸を理解することが不可欠です.
  • 細胞骨格運動の関与に関するさらなる研究は,タンパク質の誤局に関連した疾患のための新しい治療標的を明らかにすることができます.