ポリコンブドメインの遺伝子バイバル性は,頭蓋骨神経頂部位置的アイデンティティを調節する

Maryline Minoux^1,2, Sjoerd Holwerda¹, Antonio Vitobello¹

¹Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4051 Basel, Switzerland.

Science (New York, N.Y.)

|April 1, 2017

まとめ

頭蓋骨の神経頂部細胞

科学分野:

発達生物学
エピジェネティクス
ゲノミクス

背景:

頭蓋の神経の細胞は頭蓋の発達に不可欠です
顔の形態変異を解読する鍵となるのは顔の表情学的調節を理解することです

研究の目的:

ネズミの頭蓋骨神経の亜集団のクロマチンの風景を調査する.
頭蓋骨の神経頂部細胞のアイデンティティにおける表遺伝子マークの役割を明らかにする.

主な方法:

クロマチンのアクセシビリティの分析 in vivo
H3K27me3とH3K4me2のためのクロマチン免疫降水配列 (ChIP-seq)
細胞サブ集団の転写プロファイリング

主要な成果:

移住後の頭蓋骨神経頂部亜集団は,類似のクロマチンのアクセシビリティを示したが,転写的に異なっていた.
ポリコンブドメイン内の差異的に静止された遺伝子で二重染色体マーク (H3K27me3/H3K4me2) が発見された.
これらの二価領域は移住前の祖先の初期に確立された.

結論:

双対ポリコンドメインは,頭蓋骨の神経頂部細胞の位置的アイデンティティを調節するクロマチンのテンプレートとして機能する.
エピジェネティックメカニズム,特にポリコンブ群のタンパク質は,顔の形態変異に不可欠です.

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