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関連する概念動画

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this defense.
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

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関連する実験動画

Updated: Jun 15, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

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CRISPR-Cas13a/C2c2による核酸検出

Jonathan S Gootenberg1,2,3,4,5, Omar O Abudayyeh1,2,3,4,6, Jeong Wook Lee7

  • 1Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|April 15, 2017
PubMed
まとめ

新しいCRISPRベースの診断装置 (CRISPR-Dx) SHERLOCKは 迅速で高度に敏感な核酸検出を可能にします この分子ツールで 特定のウイルスや細菌 変異を特定できます フィールド環境でもです

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Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
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Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

関連する実験動画

Last Updated: Jun 15, 2026

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents
10:16

Point-of-care CRISPR-based Diagnostics with Premixed and Freeze-dried Reagents

Published on: August 16, 2024

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

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科学分野:

  • 分子生物学
  • バイオテクノロジー
  • 遺伝学

背景:

  • 核酸検出は,診断,病原体識別,および疾患モニタリングに不可欠です.
  • CRISPR-Cas13aシステムは,付随の酵素活性でRNA標的化能力を提供しています.
  • 現存する方法は,診療所での適用に必要な速度,感度,または特異性を欠いている可能性があります.

研究 の 目的:

  • 迅速で,安価で,非常に敏感な核酸検出プラットフォームを開発する.
  • 診断目的でCas13aの付随活動を活用する.
  • 病原体検出,遺伝子型決定,変異識別のための多用途ツールを作成する.

主な方法:

  • 結合されたCas13aの付随リボヌクレアース活性と同熱増幅.
  • CRISPRベースの診断プラットフォームを開発し,SHERLOCK (特定高感度酵素レポーター解鎖) と名付けました.
  • 特定のウイルス株 (ジカ,デング熱),細菌,ヒトDNA,細胞フリー腫瘍DNA変異の検出に SHERLOCK を使用した.

主要な成果:

  • 核酸検出でアトモラー感度と単塩基不一致特異性を達成した.
  • ウイルス,細菌,ヒトのDNAを含む様々な標的を検出する能力を示した.
  • 冷凍連鎖の独立性および紙上のフィールド展開のための冷凍反応剤の潜在性を示しました.

結論:

  • SHERLOCKはCRISPRベースの強力な診断ツールで 迅速かつ敏感な分子検出を可能にします
  • このプラットフォームの汎用性は,病原体識別,遺伝子分析,癌変異検出に及ぶ.
  • 溶融化と紙ベースの再構成は,アクセシブルで,ケアポイントで,フィールドベースのアプリケーションをSHERLOCKに可能にします.