HIV-2およびSIVに特異的な自然免疫性ビリオン関連タンパク質です
1Department of Cancer Biology, Harvard University School of Public Health, Boston, Massachusetts 02115.
Nature
|September 15, 1988
まとめ
HIV-2とSIVのオープン・リーディング・フレーム-x (orf-x) は,HIV-1には存在しない. その遺伝子製品は,HIV-2をHIV-1感染から区別するのに役立つ,in vitro複製のために必要不可欠です.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 分子生物学は分子生物学である.
- 免疫学 免疫学とは
背景:
- ヒト免疫不全ウイルス1型 (HIV-1),HIV2型 (HIV-2),猿類免疫不全ウイルス (SIV) は,ゲノムの類似性を共有しています.
- 開いた読み取りフレーム,orf-xはHIV-2とSIVに存在するが,HIV-1には存在しない.
- orf-xにおける配列保存は,ウイルスの感染性または複製における潜在的な役割を示唆しています.
研究 の 目的:
- SIVとHIV-2の複製におけるorf-xの役割を調査する.
- orf-x製品がバイリオンに存在するかどうかを判断する.
- orf-xとその抗体の診断の可能性を評価し,HIV-2とHIV-1の感染を区別する.
主な方法:
- orf-x遺伝子に早期停止コドンの導入.
- インビトロ感染性および複製性アッセイ.
- HIV-2およびSIV陽性血清サンプルおよびSIV陽性サル血清を用いた抗体検出アッセイ.
主要な成果:
- SIVMAC の orf-x 製品と HIV-2SBL-6669 は,ウイルスと関連していることが判明しました.
- orf-xをストップコドンで破壊することは,ウイルス感染力や複製に in vitro 影響を及ぼさなかった.
- orf-x製品に対する抗体は,HIV-2およびSIV陽性サンプルの大部分で検出されたが,HIV-1陽性サンプルまたは対照群では検出されなかった.
結論:
- orf-x遺伝子は,SIVMAC.のインビトロ複製に欠かせない.
- HIV-2 の orf-x 遺伝子産物またはそれに対する抗体は,HIV-2 をHIV-1 感染から区別するためのマーカーとして機能することができます.
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