多剤流出ポンプAcrAB-TolCの偏った分割は,長寿命の異質性に基づいています

Tobias Bergmiller¹, Anna M C Andersson¹, Kathrin Tomasek¹

¹Institute of Science and Technology Austria, 3400 Klosterneuburg, Austria.

Science (New York, N.Y.)

|April 22, 2017

まとめ

Escherichia coliの主要な多剤流出ポンプであるAcrAB-TolCは,古い細胞極に分離する. これは母細胞と子細胞の異なった表型につながり,細菌の成長と多剤耐性に影響を与えます.

科学分野:

バクテリア生理学
分子微生物学
人口動態

背景:

同型細菌集団におけるフェノタイプの変動はよく理解されていません.
マルチドラッグ・エフフルスポンプは抗生物質耐性を引き起こす
細胞の非対称性は表型分岐に繋がる

研究の目的:

エシェリキア・コライの表型変化の分子メカニズムを調査する.
細胞の異質性を生み出すAcrAB-TolC流出ポンプの役割を明らかにする.
流出ポンプの分割が細菌の増殖と薬剤耐性をどう影響するかを理解する

主な方法:

生細胞画像と数学的モデルを使用した.
エシェリキア・コリ菌における AcrAB-TolC 流出ポンプの分割を調査した.
偏った流出ポンプ分離の現象的結果を分析した.

主要な成果:

AcrAB-TolC 流出ポンプは,三重複合体の形成により,古い細胞の極に強い分割バイアスを表しています.
古いポールを継承する母細胞は,子細胞と比較して薬の放出活性が増加しています.
系統的で持続的な成長率の差異は,抑制性抗生物質濃度下で母細胞と子細胞の間に発生する.

結論:

AcrAB-TolC流出ポンプの偏った分割は,細菌集団内で長寿で異質なフェノタイプを生成します.
このメカニズムは,抑制性抗生物質レベルでも,持続的な成長率の違いに貢献します.
この発見は,バクテリアの多剤耐性の発生と持続を理解する上で重要な意味を持つ.

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