エンジニアリング 制御 システム は,腸内 の 人 の 配偶 者 の 遺伝子 表達 を 調節 する
Bentley Lim1, Michael Zimmermann1, Natasha A Barry1
1Department of Microbial Pathogenesis and Microbial Sciences Institute, Yale University School of Medicine, New Haven, CT 06536-0812, USA.
Cell
|April 22, 2017
まとめ
研究者はバクテロイド菌のための新しい誘導性遺伝子発現システムを開発した. このツールは腸内の遺伝子活動を正確に制御し,マイクロバイオームと宿主の相互作用に関する新しい研究を可能にします.
科学分野:
- 微生物学
- 分子生物学
- システム生物学
背景:
- 腸内微生物群は 健康や病気に 重要な役割を果たします
- 腸内無酸素菌に対する 限られた遺伝的ツールは 微生物群の機能に関する研究を妨げています
- 誘導性プロモーターは,微生物の遺伝子寄与のリアルタイム分析に不可欠です.
研究 の 目的:
- 主要な腸内細菌系バクテロイドの 調整可能な遺伝子発現システムを開発する.
- 腸内でのバクテロイドの遺伝子発現のダイナミックな調節を可能にします
- 微生物酵素と宿主-病原体の相互作用の関係を調査する.
主な方法:
- バクテロイド種の 合成誘導体制御表現プラットフォームを設計した
- 誘導体と急速な誘導体 (4〜5の大きさ) の不在で完全な抑制が示された.
- マウスの腸内の遺伝子発現を調節するために,飲料水を介して誘導剤を投与した.
主要な成果:
- バクテロイドの遺伝子発現システムを in vivoで成功裏に確立した.
- 腸内での遺伝子発現を 動的に制御する能力を示した
- シアリック酸の放出に起因するシアリダース活性への影響を研究するために,このシステムを利用した.
結論:
- 開発された誘導表現システムは,腸内のバクテロイドを研究するための強力なツールを提供します.
- このプラットフォームは,マイクロバイオームの遺伝子機能と宿主の生理学と病気への影響をリアルタイムで分析します.
- 宿主-微生物-病原体のダイナミクスを探求するための新しい道を開く.
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