酵母アルファ因子受容体のカルボキシ末端セグメントは,規制ドメインである
J E Reneke1, K J Blumer, W E Courchesne
1Department of Biochemistry University of California, Berkeley 94720.
Cell
|October 21, 1988
まとめ
アルファファクターの受容体
科学分野:
- 細胞生物学 細胞生物学
- イーストの信号伝達経路の分子および遺伝学的研究.
背景:
- アルファ因子受容体は,フェロモン曝露時に急速に高酸化される.
- 変異したC端末ドメインを持つ変異受容体は生物学的に活性ですが,過敏症と回復障害を示します.
研究 の 目的:
- アルファ因子受容体の調節と適応におけるC末端ドメインの役割を調査する.
- 酵母におけるフェロモン反応適応のメカニズムを理解するために.
主な方法:
- 変異したC端末ドメインを持つ変異したアルファ因子受容体の構築と分析.
- 受容体活性,リガンド結合,エンドサイトーシスの評価.
- SST2機能を含む遺伝子解析.
主要な成果:
- C末端領域に欠けているか,または変化している変異性受容体は,過敏症と成長停止からの回復の欠陥を示します.
- 部分的なC端末切断は,リガンド誘発性エンドサイトーシスにつながり,複雑な適応メカニズムを示しています.
- C端末ドメインの完全な削除は,リガンド誘発性エンドサイトーシスを廃止します.
- SST2媒介による適応は,受容体のC端末領域に依存しない.
結論:
- アルファ因子受容体のC端領域は,フェロモンシグナル伝達からの適応と回復において重要な役割を果たします.
- エンドサイトーシスによる受容体のダウンレギュレーションは,重要な適応メカニズムですが,唯一のメカニズムではありません.
- 適応におけるSST2機能は,α因子受容体のC端末領域とは独立している.
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