cdc2はM相特異ヒストンH1キナーゼの成分である:MPFとの同一性の証拠
D Arion1, L Meijer, L Brizuela
1CNRS, Roscoff, France.
Cell
|October 21, 1988
まとめ
海星卵細胞の特定のヒストンH1キナーゼ (H1K) は,中性分裂の間に活性化されます. このM相特異のH1Kは,cdc2タンパク質を含んでおり,M相促進因子 (MPF) と同じであることを示唆しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
背景:
- M相特異ヒストロンH1キナーゼ (H1K) の活性が,真核細胞全体で観察されている.
- 海星卵細胞では,1-メチラデニンは,H1Kの活動が著しく急激に増加する同時中性分裂を誘発する.
研究 の 目的:
- 海星卵細胞から活性化されたH1Kを実質的に浄化し,特徴づけること.
- 活性化されたH1Kが,既知の細胞周期調節タンパク質と関連しているかどうかを判断する.
主な方法:
- 酵素浄化と活性測定法.
- 分裂酵母に対する抗体を用いた定量免疫ブロッティング cdc2.
- 分子サイズのクロマトグラフィー. 分子サイズのクロマトグラフィー.
- p13-セファロースによる結合測定法.
主要な成果:
- 精製された海星H1Kは34 kDaのタンパク質に富んでいる.
- 34 kDaのタンパク質は,酵母cdc2.2.に対する抗体とクロス反応する.
- 海星H1Kはcdc2ホモログと共解し,p13-セファロースと結合する.
- H1Kの活性化運動は,MPFの活性化運動と一致する.
結論:
- cdc2ホモログは,海星介質卵細胞におけるM相特異のH1Kのサブユニットである.
- ヒストンH1キナーゼ (H1K) とM相促進因子 (MPF) はおそらく同じ存在である.
- ヒストンH1は,プレオトロプ的MPFの潜在的な基質である.
さらに関連する動画
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関連する概念動画
01:45Positive Regulator Molecules
To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.
02:39Positive Regulator Molecules
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...