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関連する概念動画

Histone Modification02:32

Histone Modification

16.7K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.7K
Histone Modification02:32

Histone Modification

4.7K
4.7K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

7.8K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
7.8K
Heterochromatin02:38

Heterochromatin

18.9K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...
18.9K
Heterochromatin02:38

Heterochromatin

4.9K
4.9K
Euchromatin01:01

Euchromatin

9.2K
The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
9.2K

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Analysis of Histone Antibody Specificity with Peptide Microarrays
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Analysis of Histone Antibody Specificity with Peptide Microarrays

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遺伝的にコードされたフォトアフィニティ ヒストンマーク

Xiao Xie1, Xiao-Meng Li2, Fangfei Qin3,4

  • 1Synthetic and Functional Biomolecules Center, Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University , Beijing 100871, China.

Journal of the American Chemical Society
|May 2, 2017
PubMed
まとめ
この要約は機械生成です。

研究者はヒストンのクロトニル化 (Kcr) を研究するための新しいツールを開発し,これは重要な翻訳後の修正である. このフォトアフィニティ・プローブは,Kcrの調節に関与する酵素やタンパク質を特定し,その生物学的役割の理解を深めることができます.

さらに関連する動画

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue

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関連する実験動画

Last Updated: Mar 3, 2026

Analysis of Histone Antibody Specificity with Peptide Microarrays
09:47

Analysis of Histone Antibody Specificity with Peptide Microarrays

Published on: August 1, 2017

42.0K
Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
10:28

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Global Level Quantification of Histone Post-Translational Modifications in a 3D Cell Culture Model of Hepatic Tissue
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科学分野:

  • 生物化学
  • 分子生物学
  • エピジェネティクス

背景:

  • ヒストンのライシン残留の翻訳後の改変 (PTM) は重要な表遺伝子調節因子である.
  • クロトニル化などの新発見されたライシンPTMの機能的意義と規制メカニズムは完全に解明されていません.
  • これらの変化を理解することは 遺伝子調節と細胞過程の解読に不可欠です

研究 の 目的:

  • ヒストンライシンクロトニレーション (Kcr) の調査のための新しい化学生物学ツールを開発する.
  • サイト特有のKcrアナログをヒストンに組み込むことを可能にする.
  • ヒストンのクロトニル化に責任を負う酵素機構とエフェクタータンパク質の識別を容易にする.

主な方法:

  • フォトアフィニティクロトニルライシン (Kcr) アナログの設計と合成
  • Kcr アナログとフォトライシン制御プローブをヒストンタンパク質に組み込む.
  • 相互作用するタンパク質を捕捉し識別するために,質量スペクトロメトリーに基づくプロテオミクスを用いる.

主要な成果:

  • フォトアフィニティのKcrアナログをヒストンに成功裏に組み込む.
  • ヒストンのクロトニル化に関連したタンパク質を捕捉し,識別する探査機の有用性の実証.
  • Kcr経路に関与する潜在的な酵素およびエフェクタタンパク質の特定.

結論:

  • 開発されたフォトアフィニティKcrアナログは,ヒストンのクロトニル化を研究するための強力なツールです.
  • このアプローチにより,Kcrを調節する新しいタンパク質の発見が可能です.
  • リジンPTMによる表遺伝的調節とその関連する生物学的機能の理解を進める.