リピッドバイヤーにおける信号伝達のための認識制御された膜転移
Matthew J Langton1, Nicholas H Williams2, Christopher A Hunter1
1Department of Chemistry, University of Cambridge , Lensfield Road, Cambridge CB2 1EW, United Kingdom.
Journal of the American Chemical Society
|May 3, 2017
まとめ
合成小胞は 金属イオンを使って 信号を発信できます 研究者らは 銅イオンが 膜トランスデューサを制御し 内部の触媒と光出力を オン・オフするシステムを設計しました
科学分野:
- 生物化学
- 合成生物学
- 材料科学
背景:
- 膜シグナル伝達タンパク質は細胞膜を通して細胞外信号を伝達する.
- 超膜信号伝達のための合成システムの開発は,反応性のある人工膀の鍵です.
- 金属カチオン結合イベントは,そのようなシグナリングを開始するための制御可能なメカニズムを提供します.
研究 の 目的:
- 金属カチオン結合で制御される合成の水泡ベースのシグナルシステムを設計し,実証する.
- 分子結合のイベントと 膜伝達器の活動を組み合わせる
- カプセル化された触媒を介して制御可能で増幅された信号出力を達成する.
主な方法:
- 合成トランスデューサを 膀膜に埋め込んだ
- 銅イオンとEDTAの競争的結合を利用して,脂質二重層のトランスデューサー転移を制御した.
- カプセル化された触媒の活性化に結合したトランスデューサーの転位
- サブストラットの回転から放大された光出力を監視する.
主要な成果:
- 合成トランスデューサのメタルカチオン制御されたトランスロケーションが,脂質二重層で実証された.
- この転位が 膀内の触媒活性化と結合することを示した.
- 外部銅イオンとEDTAを用いた内部触媒と光信号の可逆性オン/オフを実現した.
- 特定の結合に反応して 増幅された光信号を生成する.
結論:
- 金属イオンの濃度に対応する合成バシクルベースのシグナルシステムは,成功裏に開発されました.
- このシステムは,分子結合によって開始される制御可能なトランスメブラン信号を可能にします.
- リバーシブルスイッチング機能は 洗練された人工セルラーシステムを 作る可能性を秘めています
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