SLERTは,Pol Iトランスクリプションに関連するDDX21リングを調節する
Yu-Hang Xing1, Run-Wen Yao1, Yang Zhang1
1State Key Laboratory of Molecular Biology, Shanghai Key Laboratory of Molecular Andrology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China.
Cell
|May 6, 2017
まとめ
新しい長い非コーディングRNAであるSLERTは,RNAポリメラーゼIの転写を調節することによって,リボソームの生体生成を強化する. SLERTとDDX21の相互作用は細胞増殖と腫瘍形成に影響する.
科学分野:
- 分子生物学
- 細胞生物学
- 癌 研究
背景:
- RNAポリメラーゼI (Pol I) によって制御不能なrRNA合成は,制御不能な細胞増殖と癌の特徴である.
- リボソームの生体生成の正確な調節は 細胞の成長と機能に不可欠です
研究 の 目的:
- リボソーム前RNA (リボソーム前RNA) 転写の調節に関与する新しい長いノンコーディングRNA (lncRNA) を特定し,特徴づけること.
- この lncRNA が Pol I の活動を制御するメカニズムとその腫瘍形成への影響を解明する.
主な方法:
- SLERT (pre- rRNA転写を強化するsnoRNA末端の長いノンコーディングRNA) の識別と機能的特徴付け.
- rRNA前転写,rRNA生成,および削除後の腫瘍形成におけるSLERTの役割の分析.
- 超解像度画像と生化学的測定を用いて,SLERTとDEAD-box RNAヘリケーズDDX21の相互作用を調査した.
主要な成果:
- SLERTは,両端にボックスH/ACAのsnoRNAがあるlncRNAであり,rRNA前転写とrRNA生成に不可欠である.
- SLERTの削除はリボソームの生殖を阻害し,腫瘍生殖を減少させます.
- SLERTはDDX21と相互作用し,Pol I複合体周辺の抑制リング構造を破壊し,それによって転写を強化する.
結論:
- SLERT lncRNAは,DDX21の活性を調節することによって,リボソームの生殖を制御する上で重要な役割を果たします.
- SLERTの規制メカニズムは,Pol Iの転写の制御に関する新しい洞察を提供し,がんに対する潜在的な治療目標を提供します.
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