レチノ酸と甲状腺ホルモンは,共通の反応性要素を通じて遺伝子発現を誘導する
Nature
|November 17, 1988
まとめ
レチノ酸受容体は,甲状腺ホルモン反応要素を介して遺伝子発現を活性化することができます. これは,脊椎動物におけるレチノ酸と甲状腺ホルモンによって調節される重複する遺伝子ネットワークを示唆している.
科学分野:
- 分子内分泌学 分子内分泌学
- 遺伝子規制 遺伝子規制
- 細胞生物学 細胞生物学
背景:
- ステロイド受容体は,甲状腺ホルモンおよびレチノ酸受容体を含む,リガンド誘導性タンパク質のスーパーファミリーを形成します.
- これらの受容体は,ホルモン反応要素と結合することによって,遺伝子発現を調節する.
- レチノ酸受容体メカニズムを理解するには,機能的なレチノ酸反応性要素を特定する必要があります.
研究 の 目的:
- レチノ酸受容体が甲状腺ホルモン反応要素を通して遺伝子発現を活性化できるかどうかを調査する.
- レチノ酸と甲状腺ホルモンのシグナル伝達経路の機能的重複を調査する.
主な方法:
- ヒトのレチノ酸受容体発現のためにクローンされた補完的なDNAを使用する.
- F9テラトカルシノーマ細胞を用いて,内生性レチノ酸受容体活性の研究.
- 自然または合成の甲状腺ホルモン反応要素と融合したプロモーターからの遺伝子発現の評価.
- ヒトのレチノ酸受容体cDNAのインビトロ翻訳を行い,DNA結合親和性を研究する.
主要な成果:
- ヒトのレチノ酸受容体 (発現または内生) は,甲状腺ホルモン反応要素からの遺伝子発現を活性化します.
- ヒトのレチノ酸受容体における in vitro 翻訳は,甲状腺ホルモン反応要素に高親和性結合を示した.
- レチノ酸と甲状腺ホルモン受容体経路の間の機能的なクロスアクティベーションが実証されています.
結論:
- レチノ酸受容体は,甲状腺ホルモン反応要素と機能的に相互作用することができます.
- この相互作用は,レチノ酸と甲状腺ホルモンが共有された遺伝子ネットワークを制御することを意味する.
- これらの重複するネットワークは,脊椎動物の発達とホメオスタシスにとって潜在的に重要である.
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