Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.8K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.8K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

10.9K
10.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

11.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
11.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

19.2K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.2K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

4.0K
4.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Multimodal Distillation and Fusion for Enhanced Age-Related Macular Degeneration Classification.

IEEE journal of biomedical and health informatics·2026
Same author

Degradation of tris-(2-chloroisopropyl) phosphate initiated by OH and ClO radicals in environment: Mechanisms, kinetics, toxicity and molecular design of substitutes.

Journal of environmental sciences (China)·2026
Same author

From mechanism to molecule: Atmospheric and aqueous degradation kinetics, toxicity assessment, and sustainable redesign of the flame retardant TDCPP.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Hangover regulates gene expression by limiting NSL-mediated H4K16 acetylation.

Nucleic acids research·2025
Same author

CMOS: Confidence-Guided Multi-Scale Semi-Supervised Segmentation for Retinal Layers in OCT Images.

Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2025
Same author

Histone modification cross-talk and protein complex diversification confer plasticity to Polycomb repression.

Genes & development·2025

関連する実験動画

Updated: Mar 2, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.5K

クリプティックプロモーターでの乱交的活性化をブロックすると,細胞タイプ特有の遺伝子発現を誘導する.

Jongmin Kim1,2, Chenggang Lu2, Shrividhya Srinivasan2

  • 1Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.

Science (New York, N.Y.)
|May 20, 2017
PubMed
まとめ

亜鉛指タンパク質であるクンガン (Kmg) とdMi-2は,ドロソフィラの雄性生殖細胞における体内遺伝子転写を阻害する. これは,望ましくないプロモーターの活動をブロックすることによって,開発中の適切な遺伝子活性化を保証します.

さらに関連する動画

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

8.6K

関連する実験動画

Last Updated: Mar 2, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
10:16

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

33.5K
Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
11:36

Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations

Published on: April 21, 2023

3.1K
Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
11:02

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1

Published on: May 27, 2016

8.6K

科学分野:

  • 発達生物学
  • 遺伝学
  • 分子生物学

背景:

  • 発現しない細胞系統の 遺伝子サイレンスを維持することは 発達過程において極めて重要です
  • 特定の転写因子とクロマチンの改造剤は,遺伝子発現パターンを調節する上で重要な役割を果たします.

研究 の 目的:

  • Kumgang (Kmg) とdMi-2がドロソフィラの雄性生殖幹細胞系における遺伝子発現を調節するメカニズムを調査する.
  • ゲノム細胞における体内遺伝子の転写を Kmg がどのように阻害するかを理解する.

主な方法:

  • ドロソフィラの雄性生殖幹細胞系統をモデルシステムとして利用した.
  • 亜鉛指タンパク質クンガン (Kmg) とクロマチンリモデレータdMi-2の機能を研究した.
  • Kmg,dMi-2とアクティベーターAlyの相互作用を分析した.

主要な成果:

  • Kmgは,dMi-2と併用して,ドロソフィラの雄性生殖細胞における体系遺伝子の転写を抑制する.
  • 不適切な遺伝子の活性化を防ぐ.
  • このメカニズムは,アクティベーターAlyが雄性生殖細胞の分化のためのトランスクリプトを特定することを保証します.

結論:

  • Kmgは特定の細胞系における遺伝子静止を維持する重要な調節剤として作用する.
  • 終端分化時に精密な遺伝子活性化のために,暗号的プロモーターの乱交活性化剤をブロックすることが不可欠です.
  • Kmg- dMi-2複合体は,体内遺伝子転写を防止することによって,生殖系統特有の遺伝子発現を保護する.