G四重体におけるリボースG四重体の位置に依存する予期せぬ効果

Jun Zhou^1,2, Samir Amrane², Frédéric Rosu³

¹State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry & Chemical Engineering, Nanjing University , Nanjing 210023, China.

Journal of the American Chemical Society

|May 20, 2017

まとめ

リボースG四重奏は,位置に依存した方法でG四重奏の安定性と構造に影響を与える. 予期せぬ不安定化が5'-endで発生し,RNA安定化の信念に挑戦した.

科学分野:

構造生物学
生物化学
核酸化学

背景:

G四重複体は様々な生物学的役割を持つ核酸構造である.
リボース置換のような変化を理解することは機能の鍵です
RNAの安定性G四重複体に関する以前の仮定は,再評価する必要がある.

研究の目的:

リボースG四重体のG四重体の安定性と構造に対する影響を調査する.
リボース置換の位置依存的効果を決定する.
リボースの組み込みによって誘発された新しいG-四重複のトポロジーを探求する.

主な方法:

異なる数のリボースG四重組置換によるDNAG四重組の合成.
G四重複の安定性を評価するための熱変性実験.
構造分析のための二次元核磁気共鳴 (2D NMR) スペクトロスコーピー.

主要な成果:

リボースG四重奏は,G四重奏の安定性に位置依存し,累積的な効果を発揮する.
予期せぬ不安定化は5段階のリボースG四重奏で観察された.
水分はキメリックG四重複の安定性の主要な要因ではなかった.
中央リボースG四重奏は,異なるCD信号を持つ新しいテトラ分子トポロジーを促進しました.
NMRは,キメリック構造の5 étaire-most G-クォーテットでGのシンコンファメーションを明らかにしました.

結論:

リボース残基の位置は G-四重複の特性を決定的に調整する.
リボースのG四重奏は,G四重奏を不安定化させることができる. 一般的な期待に反して.
この研究は,RNA/DNAキメリックG四重複体とその構造的可塑性に関する新しい洞察を示しています.

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