セルフヒーリング,セルフアセンブリドβシートペプチド-ポリ (γ-グルタミン酸) ハイブリッドヒドロゲル
David E Clarke1, E Thomas Pashuck1, Sergio Bertazzo1
1Department of Materials, ‡Institute of Biomedical Engineering, and §Department of Bioengineering, Imperial College London , Exhibition Road, London, SW7 2AZ, U.K.
Journal of the American Chemical Society
|May 20, 2017
まとめ
研究者らはポリマーと自己組み立てペプチドネットワークを使って 自己回復するハイブリッドヒドロゲルを開発しました これらのバイオマテリアルには 調整可能な機械的特性があり 組織工学や生物医学の応用に適しています
科学分野:
- バイオマテリアル科学
- ポリマー化学
- 組織工学
背景:
- 自己組み立てバイオマテリアルは注射可能であり,in situで形成されますが,生物学的用途では機械的な強度が欠けていることが多いです.
- 既存の材料は低張力では機械的整合性を失い,その使用を制限します.
- 調整可能な機械的性質と 自己修復能力を持つ先進的なバイオマテリアルが必要です
研究 の 目的:
- 強化された機械的特性と自己治癒能力を持つ新しいハイブリッドヒドロゲルを合成し,特徴づけること.
- βシートペプチドの移植密度とヒドロゲルの機械的性能との関係を調査する.
- 組織工学やバイオメディカルアプリケーションのプラットフォームとしてこれらのヒドロゲルの可能性を探求する.
主な方法:
- 合成されたハイブリッドヒドロゲルは,ポリー (γ-グルタミン酸) ポリマーネットワークを,自己組み立てのβシートペプチドで物理的にクロスリンクする.
- βシートペプチドの移植密度と濃度を変化させることで,機械的性質を調整した.
- βシートと探査機二次構造の存在を確認するために,スペクトロスコピク技術を使用した.
- 機械的な故障後の貯蔵モジュールの回復を測定することで,自己修復特性が評価される.
主要な成果:
- 柔らかい組織に匹敵する10〜200kPaの範囲の調整可能な機械性能を達成した.
- βシートクロスリンクの再組成による故障後の貯蔵モジュールを回復するヒドロゲルの有意な自己治癒能力を示した.
- ハイブリッドヒドロゲル内のβシート二次構造の存在をスペクトル解析で確認した.
- ヒドロゲル形成には15%未満の機能化が必要であり,さらなる改変が可能です.
結論:
- 開発された自己治癒ポリマー-βシートペプチドハイブリッドヒドロゲルは,柔らかい組織に適した調整可能な機械特性を持っています.
- βシートクロスリンクメカニズムは,損傷後に機械的整合性を回復する優れた自己治癒能力を提供します.
- これらのハイブリッド・ヒドロゲルは 先進的な組織工学・エスカフォルドや 生物医学的な応用のための有望なプラットフォームです
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