規制されたイントロンの除去は,動機付け状態と経験を統合します

Jason Gill¹, Younshim Park¹, J P McGinnis¹

¹Stowers Institute for Medical Research, 1000E 50(th) Street, Kansas City, MO 64110, USA; Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA.

Cell

|May 20, 2017

まとめ

長期記憶形成にはドロソフィラ・オーブ2Aタンパク質が必要です合成されていないmRNAからタンパク質をコードする形に変換する過程は,経験と内部状態を統合するパシラ遺伝子によって制御される.

科学分野:

神経科学
分子生物学
遺伝学

背景:

記憶の持続性は内部状態と経験の重要性に依存しています
経験と内在状態と記憶の持続を結びつける分子機構は不明である.
ドロソフィラの長期記憶は,Orb2Aタンパク質の特定の集合状態を必要とします.

研究の目的:

経験と内部状態が長期記憶の持続時間に影響を与える分子メカニズムを解明する.
Orb2Aタンパク質のレベルを制御する制御要因を特定する.

主な方法:

大人のハエの脳におけるOrb2A mRNAのスプライシングの分析.
Orb2A mRNA処理に関与する要因を特定するための遺伝子スクリーニング.
記憶形成におけるパシラ遺伝子の役割を調べる
サイト・ディレクテッド・ミュータジェネシスで,変化したパシラ結合が記憶に与える影響を評価する.

主要な成果:

Drosophila Orb2A mRNAは,成人の脳内では,プロテインコード化されていない形で存在します.
経験と内部の状態が合わさってタンパク質をコードするOrb2A mRNAが一時的に増加します
パシラ遺伝子 (フライ Nova-1/2 ortholog) は,Orb2A mRNA処理を媒介する.
パシラ結合とイントロンの除去を減少させる特定のイントロニック変異は,長期記憶を損なう.

結論:

Orb2A mRNAのパシラ媒介処理は,長期記憶にとって極めて重要です.
この処理は,外部の経験と内部の状態を統合して,Orb2Aタンパク質の豊富さを調節します.
内部と外部のシグナルに基づいて記憶の持続時間を制御する分子メカニズムが明らかになりました

キーワード:

ノバアミロイド拘束されたイントロンイントロン保持長期記憶動機づけパシラバランスのとれたスプライシングプリオン

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