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関連する概念動画

Spindle Assembly02:50

Spindle Assembly

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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
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Centrioles and Centrosomes01:13

Centrioles and Centrosomes

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Most animal cells comprise a pair of centrioles together called a centrosome. The cell duplicates its centrosome and contains two centrosomes side-by-side, which begin to move apart during the prophase. As the centrosomes migrate to two different sides of the cell, microtubules start extending from each centrosome toward the other end. The mitotic spindle is composed of the centrosomes and their emerging microtubules.
Near the end of the prophase, also called late prophase or...
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The Mitotic Spindle02:27

The Mitotic Spindle

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The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures...
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Centrosome Duplication02:25

Centrosome Duplication

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The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
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Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

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As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
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Microtubules in Cell Motility01:24

Microtubules in Cell Motility

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Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
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Imaging Centrosomes in Fly Testes
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ハエのミトック・センターソーム組成の構造的基礎

Zhe Feng1, Anna Caballe1, Alan Wainman1

  • 1The Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK.

Cell
|June 3, 2017
PubMed
まとめ
この要約は機械生成です。

セントロソミン (Cnn) がミトスのセントロソームで支架に組み立てられるには,そのルシンジッパー (LZ) とCnn-モチーフ2 (CM2) ドメインが必要です. これらのドメインはテトラメアを形成し,Cnnの本質的な能力が不可欠な支架を構築することを可能にします.

キーワード:
セントロソミンCnn ニュースPCM についてPlk1 についてセントリオール中心体ミトシス

さらに関連する動画

Author Spotlight: Investigating Asymmetric Cell Division Dynamics: A Protocol for Live-Imaging of Drosophila Larval Brain Explants
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets
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科学分野:

  • 細胞生物学
  • 構造生物学
  • 分子生物学

背景:

  • セントロソミン (Cnn) は,ハエのミトーシス・セントロソームにタンパク質を誘導する支架の形成に不可欠です.
  • Cnnのエスカファルドの組み立ての正確なメカニズムは,ほとんど不明のままです.

研究 の 目的:

  • セントロソミン (Cnn) 構造の基礎にある分子メカニズムを解明する.
  • ミトスのセンターソーム形成に必要なCnn相互作用の構造的基礎を決定する.

主な方法:

  • タンパク質とタンパク質の相互作用を特徴づけるためのインビトロ生化学測定法.
  • レウシンジッパー (LZ) とCnn-モチーフ2 (CM2) 複合体の結晶構造の決定.
  • 改変されたLZドメインとPlk1リン酸化によるin vitro組立試験.
  • 遺伝子変異によってCnnの構造に与える影響を評価する研究.

主要な成果:

  • 脚本組立には,2:2複合体を形成する保存されたルシンジッパー (LZ) とCnn-モチーフ2 (CM2) ドメインが必要である.
  • 結晶構造は,LZとCM2タンパク質が,テトラメアに組み合わされる螺旋型ジマーを形成していることを示している.
  • in vitro の Plk1 リン酸化は,LZ と CM2 の間のマイクロンスケールアセンブリの形成を刺激する.
  • LZ:CM2テトラマー形成を阻害する突然変異は,in vitro組立体と,in vivo Cnn-スキャフォールド組立体の両方を損なう.

結論:

  • Cnn分子には,LZ:CM2の相互作用に依存する大規模なアセンブリ形成の固有の能力があります.
  • これらのLZ:CM2媒介の組み立ては,ミトスのセンターソームの適切な組み立てに不可欠です.
  • この研究は,ミトスのセンターソーム組立を制御する重要な分子相互作用に関する最初の原子レベルの洞察を提供します.