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Updated: Feb 27, 2026

Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling

Published on: September 20, 2016

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ミトコンドリアに小分子プローブを送り出し,無痕リンクを使用する

Eric K Lei1, Shana O Kelley1

  • 1Department of Biochemistry, Faculty of Medicine, §Department of Chemistry, Faculty of Arts and Science, and #Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto , Toronto, Ontario M5S 3M2, Canada.

Journal of the American Chemical Society
|July 1, 2017
PubMed
まとめ

研究者は,ミトコンドリアに浸透するペプチド (MPPs) の切断可能なリンカーを開発し,ミトコンドリア発送後に治療用荷物を放出しました. このアプローチは,直接結合による活性喪失を克服し,効果的なミトコンドリアの薬物標的化を可能にします.

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Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
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Labeling DNA Probes03:31

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Protein Transport into the Inner Mitochondrial Membrane

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Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
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Mitochondrial Precursor Proteins

Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
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