関連する実験動画
Updated: Aug 17, 2026

08:54
An Immunofluorescent Method for Characterization of Barrett’s Esophagus Cells
Published on: July 20, 2014
ヒトアデノカルシノーマにおけるc-erbB-2オンコゲンの増幅 in vivo
Lancet (London, England)
|April 5, 1986
まとめ
c-erbB-2遺伝子は,いくつかのアデノカルシノーマで増幅され,その発達の役割を示唆しています. 皮質成長因子 (EGF) 受容体遺伝子 (c-erbB-1) は,少数の状細胞癌で増幅されています.
科学分野:
- 分子生物学は分子生物学である.
- 腫瘍学 腫瘍学
- 遺伝学 遺伝学とは
背景:
- 人間のゲノムには,ウイルスErbB遺伝子に関連する2つの遺伝子:c-erbB-1 (表皮成長因子受容体) とc-erbB-2が含まれています.
- c-erbB-2は,EGF受容体と異なる受容体型のタンパク質をコードする.
研究 の 目的:
- 人間の悪性腫瘍におけるc-erbB-2およびc-erbB-1/EGF受容体遺伝子の増幅を調査する.
- 特定の癌のタイプにおけるこれらの増幅遺伝子の潜在的な役割を決定する.
主な方法:
- DNAハイブリッド化分析は,ヒトの新鮮な悪性腫瘍101本で行われました.
- 腫瘍は,c-erbB-2およびc-erbB-1/EGF受容体の遺伝子増幅を分析した.
主要な成果:
- c-erbB-2遺伝子は,63種のうち5種のアデノカルシノーマで増幅された.
- 他の38種類の腫瘍では,c-erbB-2の増幅は認められなかった.
- c-erbB-1/EGF受容体遺伝子は,8つの状細胞がんのうち1つで増幅された.
結論:
- c-erbB-2遺伝子の増幅は,アデノカルシノーマの進化に役割を果たす可能性があります.
- EGF受容体遺伝子は,いくつかの状細胞癌の発生に関与している可能性があります.
さらに関連する動画
09:38Establishing Dual Resistance to EGFR-TKI and MET-TKI in Lung Adenocarcinoma Cells In Vitro with a 2-step Dose-escalation Procedure
Published on: August 11, 2017
03:55Robust Detection of Gene Amplification in Formalin-Fixed Paraffin-Embedded Samples by Fluorescence In Situ Hybridization
Published on: July 12, 2024
関連する概念動画
Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Ras Gene
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a superfamily...
Ras is a superfamily...
mTOR Signaling and Cancer Progression
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
The Retinoblastoma Gene
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...