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関連する概念動画

CRISPR01:59

CRISPR

58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

808
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
808
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
Homologous Recombination02:31

Homologous Recombination

64.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.3K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.0K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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関連する実験動画

Updated: Feb 26, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

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CRISPRゲノム統合複合体の構造

Addison V Wright1, Jun-Jie Liu1,2, Gavin J Knott1

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720, USA.

Science (New York, N.Y.)
|July 22, 2017
PubMed
まとめ

Cas1-Cas2インテグラーゼは 配列だけではなく DNAの構造を使って 異なったDNAを CRISPRの位置に統合します このメカニズムは,IHFによって支援され,ウイルスに対する正確な細菌の適応免疫を保証します.

さらに関連する動画

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

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関連する実験動画

Last Updated: Feb 26, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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科学分野:

  • 分子生物学
  • 微生物学
  • 構造生物学

背景:

  • CRISPR-Casシステムは細菌にファグに対する適応免疫を提供します.
  • Cas1-Cas2インテグラーゼは,新しいディスペーサーを取得し,外来DNAをCRISPRロカスに統合するために不可欠です.

研究 の 目的:

  • Cas1-Cas2インテグラーゼによるDNA統合の構造的メカニズムを解明する.
  • CRISPR配列の拡張で統合場所の選択がどのように達成されるかを理解する.

主な方法:

  • Cas1-Cas2-DNA複合体の構造を決定するX線結晶学.
  • IHFとCRISPRの整合複合体のための冷凍電子顕微鏡

主要な成果:

  • 結晶構造は,Cas1-Cas2がドナーと標的DNAに結合することを示しています.
  • Cryo-EM構造は,統合宿主因子 (IHF) を含む統合複合体を示しています.
  • 統合部位の選択は,間接的なDNA配列認識と配列依存のDNA変形によって決定される.

結論:

  • Cas1-Cas2は,サイト固有の統合のために,繰り返しおよび側面配列変形を好むDNAの構造特性を利用します.
  • IHF結合は,上流モチーフをCas1と接触させることで,統合の特異性と効率性を高め,鋭いDNA曲線を誘導する.
  • これらの発見は,細菌の適応免疫における部位選択的CRISPR配列拡張のためのCas1-Cas2による配列依存DNA構造認識のメカニズムを説明する.