関連する実験動画
Updated: Feb 25, 2026
11:53
Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
28.1K
Cas13aによるRNA誘導RNA分裂の分子構造
Liang Liu1, Xueyan Li2, Jun Ma3
1Key Laboratory of RNA Biology, CAS Center for Excellence in Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Cell
|August 1, 2017
まとめ
CRISPR- Cas13a酵素は,強力なRNA誘導リボヌクレアースであり,標的RNA結合によって活性化されます. この構造研究は,Cas13aがRNAを分割する方法を明らかにし,RNA操作技術への道を切り開きます.
科学分野:
- 分子生物学
- 生物化学
- 遺伝学
背景:
- CRISPR-Cas13aはRNA技術に潜在力を備えたRNA誘導リボヌクレアースである.
- 活性化メカニズムの理解は 能力を活用するために不可欠です
研究 の 目的:
- Cas13aの活性化とRNAの分裂の構造的基礎を解明する.
- 標的RNA結合時にCas13aの形状の変化を調査する.
主な方法:
- Leptotrichia buccalis (Lbu) Cas13aがcrRNAと標的RNAに結合する結晶構造の決定
- LbuCas13a-crRNA複合体の冷凍電子顕微鏡 (冷凍EM)
主要な成果:
- crRNA-標的RNA複合体は,核糖節の中央チャネル内に結合する.
- 標的RNAの結合は,Cas13aにおける重要な形状変化を誘導する.
- 誘導標的RNA複合体の形成は,RNA分裂のためのHEPN触媒ドメインを活性化します.
結論:
- Cas13aの活性化には,ガイド・ターゲットRNAの二重複形成によって引き起こされる構造的シフトが含まれます.
- 活性化された酵素は標的RNAと付随RNAの両方を割ります.
- これらの発見は,RNAファージに対するタイプVIのCRISPR-Casシステムの防御機構を明らかにし,RNA操作ツールの開発を促します.
関連する概念動画
01:23RNA Interference
28.2K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
28.2K
02:53CRISPR and crRNAs
19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
02:47Ribozymes
13.5K
The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can...
Ribozymes can...
13.5K
01:19RNA Structure
7.9K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
7.9K
01:23RNA Structure
79.4K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.4K
01:59CRISPR
58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K