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¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
Chemical Ionization (CI) Mass Spectrometry01:21

Chemical Ionization (CI) Mass Spectrometry

The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
Spectroscopy of Carboxylic Acid Derivatives01:26

Spectroscopy of Carboxylic Acid Derivatives

Infrared spectroscopy is primarily used to determine the types of bonds and functional groups. In carboxylic acid derivatives, a typical carbonyl bond absorption is observed around 1650–1850 cm−1. For esters, the absorption is recorded at around 1740 cm−1, while acid halides show the absorption at about 1800 cm−1. Another acid derivative, the acid anhydrides, exhibit two carbonyl absorption around 1760 cm−1 and 1820 cm−1, arising from the symmetrical and unsymmetrical carbonyl vibration.
In the...
Mass Spectrometry: Complex Analysis01:21

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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Double Resonance Techniques: Overview01:12

Double Resonance Techniques: Overview

Double resonance techniques in Nuclear Magnetic Resonance (NMR) spectroscopy involve the simultaneous application of two different frequencies or radiofrequency pulses to manipulate and observe two distinct nuclear spins. One important application of double resonance is spin decoupling, which selectively suppresses coupling with one type of nucleus while observing the NMR signal from another nucleus, simplifying the spectrum and enhancing resolution.
Spin decoupling is usually achieved by...

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多価多機能グライカン-量子ドットを用いた多価レクトン-炭水化物認識の解剖

Yuan Guo, Inga Nehlmeier1, Emma Poole

  • 1Infection Biology Unit, German Primate Center , Kellnerweg 4, Gottingen 37077, Germany.

Journal of the American Chemical Society
|August 9, 2017
PubMed
まとめ

密度の高い砂糖配列を持つ量子ドット (QD) は,多価タンパク質-グリカン相互作用の強力な探査機として機能します. これらのグリカン-QDは,レクチン結合モードを解剖し,親和性を定量化することで,新しい抗ウイルス戦略の設計に役立ちます.

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科学分野:

  • 生物化学
  • ナノテクノロジー
  • ウイルス学

背景:

  • 多価タンパク質と炭水化物の相互作用は,最初のウイルス/細菌と細胞の接触と感染において極めて重要です.
  • 柔軟で複雑な細胞表面タンパク質に関する構造データがないため,効果的な多価抑制剤の設計が困難である.
  • DC-SIGNやDC-SIGNRのようなレクチンは HIVやエボラウイルスのウイルス侵入増殖に 重要な役割を果たします

研究 の 目的:

  • 多価タンパク質とグリカンの相互作用のための探査機として単糖/二糖質の密集した配列を表示する量子ドット (QD) を開発する.
  • これらのグリカン-QDを使用して,結合モードを解剖し,DC-SIGNとDC-SIGNRの親和性を定量化します.
  • DC-SIGN媒介によるウイルスの侵入を阻害するグリカンQDの可能性を評価する.

主な方法:

  • モノ/ディサカライドの密集した配列で機能する量子ドット (QD) の合成.
  • フォースター共振エネルギー伝送 (FRET),水力動的サイズ測定,伝送電子顕微鏡 (TEM) を組み合わせて,QD-レクチン相互作用を分析する.
  • QDとDC-SIGN/DC-SIGNRとの間の結合親和を定量化するためのQD-FRET方法の開発.

主要な成果:

  • Glycan-QDは,DC-SIGNとDC-SIGNRのテトラ-,二-,および単価結合モードを成功裏に区別しました.
  • QD-FRETアッセイでは,DC-SIGNがDC-SIGNRよりも100倍以上の強度でQDと結合することを明らかにした.
  • Glycan- QDsは,DC- SIGN媒介によるEBOV- GP伝導の強力な阻害を示し,IC50値は0. 7nMまで低かった.

結論:

  • グライカンを表示するQDは,複雑な多価タンパク質-リガンド認識を解剖するための多用途のツールです.
  • 開発されたQD-FRET方法は,結合親和性を正確に定量化し,レクチン結合モードを区別する.
  • グリカンQDは,細胞レベルで特定のタンパク質とグリカンの相互作用を標的として,ウイルス感染の効果的な阻害剤として有望であることが示されています.