トランス・ゴルギ産のエキソサイトコーティングベシクルは,新たに合成されたコレネステラーゼと内蔵されたトランスファーリンの両方を含んでいる
Cell
|January 16, 1987
まとめ
この研究では,ラットの肝細胞におけるトランスファーリン (Tf) の動きを,新しい方法を用いて追跡しています. 結果は,Tfが,細胞表面輸送におけるそれらの役割を示す,トランス・ゴルギから生じる,内細胞性水泡から外細胞性水泡へと移動することを示しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 肝臓病理学 肝臓病理学
背景:
- トランスファーリン (Tf) は,鉄を細胞に輸送するのに不可欠です.
- 内細胞および外細胞経路は,細胞内交通を媒介する.
- コーテッドベシクル (CVs) は,重要な輸送中介物質です.
研究 の 目的:
- 内部化されたトランスファーリン (Tf) の細胞内密輸を調査する.
- ネズミの肝臓におけるエクソサイトコーテッド膀 (CVs) の起源と経路を決定する.
- 輸送の移転におけるCVの役割を明らかにする.
主な方法:
- コリネステラゼ媒介密度シフトプロトコルを使用しました.
- 従業員は,内部化と動きを追跡するために125I-Tfの無線ラベルを貼った.
- ネズミの肝臓にアシアロトランスフェリンを注入して,膀の起源を調査する.
主要な成果:
- 125I-Tfは最初は内細胞性CVではなく,外細胞性CVで見つかった.
- 125I-Tfは,内部化後1〜2時間間のエクソサイト性CVに現れました.
- エクソサイト性CVには再アリルされたTfが含まれ,トランスゴルギの起源を確認した.
- エクソサイト性CVにおけるTfの動態は,細胞外放出と一致した.
結論:
- コーテッドベシクル (CVs) は,トランスファーリン輸送に関与しています.
- トランス・ゴルギネットワークは,Tf.を携えたエクソサイト性CVの起源である.
- エキゾサイト性CVは,トランスゴルギから細胞表面へのTfの転送を媒介する.
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