mRNA 3'ウリジレーションとポリー (A) 尾長が哺乳類の母性トランスクリプトームを彫刻する
Marcos Morgan1,2, Christian Much1,2, Monica DiGiacomo2
1MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh, Edinburgh EH16 4UU, UK.
Nature
|August 10, 2017
まとめ
TUT4とTUT7による末端尿解は,マウスの卵細胞における母性トランスクリプトームの形成に不可欠であり,適切な発達と生育を保証する. このプロセスは,早期の胚性プログラミングに不可欠な,トランスクリプトの除去とポリー (A) 尾の長さを調節します.
科学分野:
- 発達生物学
- 分子生物学
- 遺伝学
背景:
- オオゲネシス中に形成された母親のトランスクリプトームは 初期の胚の発達を指揮する.
- 母親のトランスクリプトーム含有量とトランスクリプト用量を制御するメカニズムは,まだ完全に理解されていません.
研究 の 目的:
- マウスの母性トランスクリプトームの形成における3'端の尿縮化の役割を調査する.
- TUT4とTUT7の作用と生殖能力の決定
主な方法:
- 尾の長さと尿化を含むmRNA 3'端の変異の分析
- ワイルド型とTUT4/TUT7欠乏性卵細胞と体細胞の比較トランスクリプトミア分析
- TUT4 と TUT7 が欠けているマウスの卵細胞の成熟と生育の評価
主要な成果:
- TUT4とTUT7によって媒介される3'末端尿解は,マウスの卵細胞成長中に特定のトランスクリプトを排除する.
- TUT4とTUT7は卵子の成熟と女性の生育に不可欠です.
- 卵細胞は,体細胞と比較して,より短いポリ・ア・テイルと,末端のオリゴ・ウリジレーションの割合が高くなります.
- TUT4/TUT7欠乏症は,短いポリ (A) 尾と3'ウリデリレーションの喪失で,体細胞の遺伝子発現は影響を受けない.
結論:
- 尾の長さと3'端の尿縮化は,母性トランスクリプトームの重要な調節因子である.
- TUT4とTUT7は,成功する繁殖のための機能的な母性トランスクリプトームの形成に特異的で重要な役割を果たします.
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