関連する実験動画
Updated: May 5, 2026

09:18
Spheroid Assay to Measure TGF-β-induced Invasion
Published on: November 16, 2011
20.8K
変形成長因子βが,ヒト乳がん細胞におけるホルモン調節された負の成長因子であることを示す証拠
Cell
|February 13, 1987
まとめ
変形成長因子-β (TGF-β) は,MCF-7乳がん細胞によって分泌され,成長阻害剤として作用します. 抗エストロゲン治療はTGF-βの分泌を増加させ,抗体によってブロックすることができます.
科学分野:
- エンドクリノロジー エンドクリノロジー
- 分子生物学は分子生物学である.
- がん研究 がん研究
背景:
- MCF-7などのホルモン依存性乳がん細胞は,成長因子を分泌することができます.
- 変形成長因子β (TGFβ) は,がんの生物学において複雑な役割を果たしています.
研究 の 目的:
- MCF-7乳がん細胞におけるTGF-βの分泌と役割を調査する.
- 抗エストロゲンがTGF-βの分泌と活性に影響するかどうかを判断する.
主な方法:
- MCF-7細胞は,抗エストロゲンで治療されました.
- 培養媒体のTGF-βレベルを測定した.
- 分泌されるTGF-βの生物学的活動は,成長アッセイと受容体結合を用いて評価された.
- エストロゲン受容体陰性細胞系を用いたコカルチャー実験が行われました.
- TGF-βに対する中和抗体を用いた.
主要な成果:
- MCF-7細胞は,受容体結合に競争する生物学的に活性なTGF-βを分泌する.
- 抗エストロゲン治療により,活性TGF-β分泌が8~27倍増加した.
- 抗エストロゲン誘発のTGF-βは,異なる乳がん細胞系統の成長を阻害しました.
- この成長抑制は,抗-TGF-β抗体によって逆転した.
結論:
- TGF-βは,MCF-7細胞のホルモン調節された成長阻害剤です.
- TGF-βは,乳がんにおけるオトクリンおよびパラクリン信号伝達経路で機能する可能性があります.
さらに関連する動画
08:48An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation
Published on: June 30, 2015
7.8K
06:54Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells
Published on: October 27, 2020
13.1K
関連する概念動画
Negative Regulator Molecules
32.1K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
32.1K
Mitogens and the Cell Cycle
6.3K
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
6.3K
Abnormal Proliferation
4.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.0K
mTOR Signaling and Cancer Progression
3.6K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.6K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
TGF - β Signaling Pathway
7.2K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.2K