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関連する概念動画

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Synthesis and Functions of Calcitonin00:51

Synthesis and Functions of Calcitonin

Calcitonin, a vital polypeptide hormone, regulates calcium levels within body fluids. It is released by the parafollicular cells, also known as C cells, situated in the follicular epithelium of the thyroid gland. Calcitonin responds to fluctuations in blood calcium levels and the influence of gastrointestinal hormones like gastrin and cholecystokinin.
The exact mechanisms by which calcitonin operates in calcium homeostasis remain elusive, but its significance is evident in several vital...

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関連する実験動画

Updated: May 10, 2026

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
10:50

Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

Published on: April 24, 2021

スプライスコミットメントは,カルシトニン/CGRP遺伝子発現におけるニューロン特異的な代替RNA処理を指示する.

S E Leff, R M Evans, M G Rosenfeld

    Cell
    |February 13, 1987
    PubMed
    まとめ
    この要約は機械生成です。

    カルシトニン/CGRP遺伝子の細胞特異的なRNA処理は,神経細胞と甲状腺細胞で明確なメッセンジャーRNA (mRNA) を生み出します. この研究は,神経因子がCGRP mRNA生産のための代替スプライシングとポリアデニレーションを決定することを示唆しています.

    さらに関連する動画

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    関連する実験動画

    Last Updated: May 10, 2026

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons
    10:50

    Molecular Modulation by Lentivirus-Delivered Specific shRNAs in Endoplasmic Reticulum Stressed Neurons

    Published on: April 24, 2021

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents
    08:39

    Ex Vivo Release of Calcitonin Gene-Related Peptide from the Trigeminovascular System in Rodents

    Published on: May 16, 2022

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay
    07:14

    Detection and Quantification of Calcitonin Gene-Related Peptide (CGRP) in Human Plasma Using a Modified Enzyme-Linked Immunosorbent Assay

    Published on: June 16, 2023

    科学分野:

    • 分子生物学は分子生物学である.
    • 神経科学は神経科学である.
    • 遺伝学 遺伝学とは

    背景:

    • カルシトニン/CGRP遺伝子は,細胞特異的な代替RNA処理を受けます.
    • これにより,異なるメッセンジャーRNA (mRNA) が生まれます:ニューロン内のCGRP mRNA,甲状腺C細胞内のカルシトニン mRNA.
    • 代替ポリアデニレーションとエクソンスプライシングは,トランスクリプトの多様性を駆動する重要なメカニズムです.

    研究 の 目的:

    • カルシトニン/CGRP遺伝子の細胞特異的な代替RNA処理の基礎となる規制メカニズムを調査する.
    • 神経細胞と甲状腺細胞の異なるmRNA生成に起因する因子または機構を特定する.
    • CGRP mRNA生成におけるスプライスコミットメントの規制要因の役割を調査する.

    主な方法:

    • ワイルド型および変異したカルシトニン/CGRP遺伝子の発現は,異種細胞系において.
    • 代替ポリアデニレーションとスプライシングのパターンを決定するために,結果のmRNA集団の分析.
    • 神経細胞と甲状腺細胞の発現パターンの比較研究.

    主要な成果:

    • ヘテロログ的発現は,細胞タイプによって,主にカルシトニンまたはCGRP mRNAを産生した.
    • 証拠によると,ニューロンは特定の機械や,代替スプライシング経路に影響を与える要因を持っていることが示唆されています.
    • 特定のスプライシング経路と,代替のポリ (A) サイト選択の間の相関が観察されました.

    結論:

    • ニューロンは,CGRP mRNA生産のための代替スプライシングとポリアデニレーションを指示する規制因子を発現する可能性が高い.
    • スプライスコミットメントの調節因子がプレ-mRNA構造を調節するという仮説があります.
    • この調節は,脳特異のCGRP mRNAを生成するために不可欠な暗号的なスプライスサイトを明らかにする可能性があります.