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哺乳類の循環型RNAの喪失は,miRNAの放出を引き起こし,脳機能に影響する
Monika Piwecka1, Petar Glažar1, Luis R Hernandez-Miranda2
1Laboratory for Systems Biology of Gene Regulatory Elements, Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine, Robert-Rössle-Straße 10, Berlin-Buch, Germany.
まとめ
Cdr1asのような循環型RNAは,脳の機能に不可欠です. Cdr1asの喪失は,感覚運動ゲートとシナプス伝達を阻害し,神経精神疾患におけるその役割を強調する.
科学分野:
- 神経科学
- 分子生物学
- 遺伝学
背景:
- 円形RNA (circRNAs) は哺乳類の脳に豊富に存在し,表現は保存されている.
- Cdr1asのような特定のcircRNAは,マイクロRNA (miRNA) と相互作用する.
研究 の 目的:
- 哺乳類の脳内のcircRNA Cdr1asのインビボ機能を調査する.
- 脳機能と行動におけるCdr1as- miRNA相互作用の役割を決定する.
主な方法:
- CDR1がノックアウトマウスを生み出す
- センソモーターゲートの行動分析
- シナプス伝送の 電気生理学的記録
- 脳組織におけるmiRNAと遺伝子発現の分析
主要な成果:
- Cdr1asのノックアウトマウスは神経精神疾患に関連した現象である感覚運動ゲートに障害がある.
- 不機能なシナプス伝達がノックアウト動物で観察された.
- miR-7とmiR-671の発現は,転写後の誤調でした.
- Cdr1as欠乏した脳では,Fosのような早期の遺伝子の発現が強化されたことが検出されました.
結論:
- この研究は,circRNA (Cdr1as) の in vivo 機能喪失フェノタイプを示しています.
- Cdr1asと特定のmiRNA間の相互作用は,正常な脳機能のために不可欠です.
- Cdr1asはシナプス伝達と行動の調節に重要な役割を果たし,神経精神疾患に影響を及ぼします.
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