Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

The DNA Replication Fork01:02

The DNA Replication Fork

41.4K
An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
41.4K
The DNA Replication Fork01:02

The DNA Replication Fork

18.8K
18.8K
Replication in Prokaryotes01:32

Replication in Prokaryotes

28.4K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
28.4K
Replication in Prokaryotes02:35

Replication in Prokaryotes

99.9K
Overview
99.9K
Replication in Eukaryotes02:31

Replication in Eukaryotes

206.2K
Overview
206.2K
Replication in Eukaryotes01:29

Replication in Eukaryotes

18.0K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
18.0K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Condensin and topoisomerases cooperate to relieve topological stress at stalled replication forks.

Nature communications·2026
Same author

ATM lifts the cGAS handbrake on DNA replication.

Nature cell biology·2026
Same author

Slx4 and Fun30/SMARCAD1 coordinate S-phase checkpoint regulation and replication fork protection in response to Top1-DNA crosslinks.

Nucleic acids research·2026
Same author

Transcription-Replication Conflicts and Incomplete Replication as a Cause of Micronuclei-Driven Chromoanagenesis.

Methods in molecular biology (Clifton, N.J.)·2025
Same author

Synthetic Lethal Combinations of DNA Repair Inhibitors and Genotoxic Agents to Target High-Risk Diffuse Large B Cell Lymphoma.

Hematological oncology·2025
Same author

Autocrine interferon poisoning mediates ADAR1-dependent synthetic lethality in BRCA1/2-mutant cancers.

Nature communications·2025

関連する実験動画

Updated: Feb 24, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K

トランスクリプション-複製の衝突: オリエンテーションの問題

Yea-Lih Lin1, Philippe Pasero1

  • 1Institute of Human Genetics, CNRS UMR9002 and University of Montpellier, Equipe Labéllisée Ligue Contre le Cancer, 34090 Montpellier, France.

Cell
|August 13, 2017
PubMed
まとめ

ゲノム不安定はDNA複製と転写の衝突から生じる. 研究によると,対向的な衝突ではなく,Rループを形成することで DNA複製フォークの進行を阻害します.

科学分野:

  • 分子生物学
  • 遺伝学
  • ゲノム不安定性

背景:

  • DNA複製と転写は 基本的な細胞プロセスです
  • これらのプロセス間の干渉はゲノム不安定につながる可能性があります.
  • この干渉のメカニズムを理解することは ゲノム維持を理解するために不可欠です

研究 の 目的:

  • DNA複製フォークの進行に対する複製-転写衝突の影響を調査する.
  • 対向衝突と対向衝突の影響を区別する.
  • 複製-転写の干渉の基礎となる分子機構を特定する.

主な方法:

  • 細菌とヒトの細胞におけるDNA複製フォークのダイナミクスの比較分析.
  • 複製を阻害するRNA-DNAハイブリッド (Rループ) の役割の調査.
  • 衝突の結果を研究するために遺伝学や分子生物学技術を活用する.

主要な成果:

  • DNA複製と転写のフォークの衝突はフォークの進行を大きく阻害する.
  • コディレクションの衝突はフォークの進行を同じ程度に妨げるようには見えない.
  • RNA-DNAハイブリッド (R-ループ) の形成は,ヘッド・オン衝突が複製フォークの停滞を引き起こす重要なメカニズムです.

さらに関連する動画

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.0K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

関連する実験動画

Last Updated: Feb 24, 2026

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

14.0K
Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
08:53

Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

Published on: May 2, 2025

1.0K
G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
06:40

G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome

Published on: March 22, 2018

6.3K

結論:

  • 複製と転写の衝突は ゲノム不安定の重要な原因です
  • Rループ形成は,ヘッド・オン衝突時の複製フォーク障害の重要な媒介である.
  • これらの発見はゲノム維持と 遺伝的不安定性の起源についての洞察を提供します