年老いた 幹 細胞 は ストレス に 適応 する ため に 日常 の リズム の 機能 を 再プログラム する
Guiomar Solanas1, Francisca Oliveira Peixoto1, Eusebio Perdiguero2
1Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, 08028 Barcelona, Spain.
Cell
|August 13, 2017
まとめ
高齢の幹細胞は 日々の遺伝子発現のリズムを ホメオスタシスから ストレス反応に切り替えます この適応は食事とカロリー制限の影響を受けます
科学分野:
- ゲロントロジー
- 幹細胞生物学
- クロノバイオロジー
背景:
- 大人の幹細胞は,ホメオスタシスに不可欠な日々のリズム機能を発揮します.
- これらのリズムが 歳をとるにつれ不規則になると考えられています
- 老化は幹細胞の機能と 組織修復能力の変化と関連しています
研究 の 目的:
- 生物時計の機能と老いた幹細胞の転写パターンを研究する.
- 幹細胞の昼夜リズムを 再プログラムする
- 食事とカロリー制限が 幹細胞に及ぼす年齢関連の昼夜変化に及ぼす影響を調べる
主な方法:
- 年老いたマウスの生理リズム分析
- 老いたマウスの表皮細胞と筋肉幹細胞の核心日経機構の評価.
- 振動する遺伝子の変化を特定するために,老化した幹細胞のトランスクリプトミカルプロファイリング.
- 生物時計の遺伝子操作だ
- 高脂肪食とカロリー制限を含む食事の介入は,若いマウスと高齢マウスの間で行われます.
主要な成果:
- 年老いたマウスは 行動的な昼夜リズムを維持します
- 年老いたマウスの幹細胞は 機能的なコアサーカディアンメカニズムを保持しています
- 老化した幹細胞の振動するトランスクリプトームは,ホメオスタシスからストレス反応遺伝子 (例えば,DNA損傷,オートファギー) にシフトして再プログラムされます.
- シルカディアン・クロック・コンポーネントの削除は この年齢関連再プログラミングを模倣しませんでした
- 高脂肪食ではなく カロリー制限は 古い幹細胞のトランスクリプトームの再プログラミングを 防ぎました
結論:
- 幹細胞の昼夜リズムは 老化中に失われず 再プログラムされます
- この再配線により 日中の遺伝子発現が ストレスへの適応へと変化します
- メタボリック・シグナルや 年齢に関係する特徴が 幹細胞の昼間の機能に影響します
- カロリー摂取制限は,幹細胞の年齢に関連する昼夜不調を緩和する可能性があります.
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