ゴルギ居住ガオは突起膜の動力を促進する

Gonzalo P Solis¹, Oleksii Bilousov¹, Alexey Koval¹

¹Department of Pharmacology and Toxicology, University of Lausanne, CH-1011 Lausanne, Switzerland.

Cell

|August 15, 2017

まとめ

Gαoタンパク質を含む新しいシグナル伝達経路は,細胞突起形成を調節する. この経路は種間で保存され,神経細胞の成長のためにゴルギからプラズマ膜への物質の供給を制御します.

科学分野:

細胞生物学
分子生物学
神経科学

背景:

ニューライトのような細胞突起は細胞機能に不可欠であり調整された誘導と成長が必要です
プラズマ膜 (PM) の細胞骨格の再編成は突起誘導を促し,指向された物質の供給は成長を促します.

研究の目的:

細胞突起形成におけるヘトロトリメリックGタンパク質のGαサブユニットの役割を調査する.
ゴルギからPMへの物質の配送を制御する分子機構とシグナル伝達経路を特定する.

主な方法:

PMとGolgiでGαoのローカライゼーション研究
多次元スクリーニングを使用してGαoインタラクターの識別 (>250が特定された).
KDELR → Gαo → Rab1/3シグナリング軸を様々な細胞タイプとモデル生物で調査する.

主要な成果:

GαoはPMとGolgiの両方に局限し,それぞれ突起誘導と物質伝達において異なる役割を持っています.
Gタンパク質のシグナル伝達のための新しいメカニズムであるモノメリックGαoを活性化します.
Gαoは,膀の輸送と効果タンパク質Rab1とRab3を直接調節する.

結論:

保存されたシグナリング軸 (KDELR → Gαo → Rab1/3) は,細胞突起形成に不可欠なゴルギからPMへの物質輸送を制御する.
この経路は神経細胞の増殖やその他の細胞過程において重要な役割を果たします

キーワード:

ドロソフィラ・メラノガスター Gタンパク質結合受容体ゴルギ装置ガオインタラクトーム KDEL受容体 Rab1/3 GTPases についてヘテロトリメリックGタンパク質ニューライトの増殖突起の形成膀の密輸

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