関連する実験動画
Updated: Jul 1, 2026

07:34
FISH for Pre-implantation Genetic Diagnosis
Published on: February 24, 2011
大量の逆転複製は,近隣のt複合体の逆転に対して,ヘテロジゴスな染色体間の同類の再組み合わせを可能にします
Cell
|March 13, 1987
まとめ
研究者はマウス染色体17の分子組織を研究し,野生型はtハプロタイプに存在しない逆転複製を含んでいることを発見しました. この構造的差異は,大きな逆転を経由して同類の再組み合わせを説明し,特定のtハプロタイプ染色体を形成します.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- 染色体組織は染色体組織である.
背景:
- マウス染色体17は,同類の再結合に関与する複雑な領域を宿している.
- tハプロタイプは,染色体17の大きな逆転と関連しています.
- この領域を理解することは,染色体進化と遺伝疾患の研究に不可欠です.
研究 の 目的:
- ネズミの染色体17の分子組織を明らかにし,同種の再結合に責任を持つ領域を明らかにする.
- この地域の野生型とtハプロタイプ染色体間の構造的違いを特徴づける.
- 特定のtハプロタイプ染色体につながる再結合のメカニズムを説明する.
主な方法:
- 制限断片の長さのポリモルフィスムの遺伝的マッピング.
- オーバーラップするコスミッドを用いたクローンDNAの分子特性.
- パルスフィールドゲル電泳による亜染色体制限マッピング.
主要な成果:
- 野生型染色体17は,大きな元素 (≥650kb) の逆転複製を有する.
- 染色体17のtハプロタイプ形態は,この要素の単一のコピーしか含まない.
- 特定のtハプロタイプ (th45,tAE5) は,この重複した元素の再結合によって生じる.
結論:
- 構造的な違い,野生型染色体17の逆転複製は,tハプロタイプとの同型再結合の基礎となっている.
- この再結合イベントは,特定のtハプロタイプ染色体の形成を説明する.
- この発見は,染色体再編成と進化の分子基礎についての洞察を提供します.
さらに関連する動画
関連する概念動画
Crossing Over
Unlike mitosis, meiosis aims for genetic diversity in its creation of haploid gametes. Dividing germ cells first begin this process in prophase I, where each chromosome—replicated in S phase—is now composed of two sister chromatids (identical copies) joined centrally.
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
The homologous pairs of sister chromosomes—one from the maternal and one from the paternal genome—then begin to align alongside each other lengthwise, matching corresponding DNA positions in a process called synapsis.
In order to...
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Overview of Transposition and Recombination
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
Homologous Recombination
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Crossing Over
Crossing over is the exchange of genetic information between homologous chromosomes during prophase I of meiosis I. Genetic recombination gives rise to allelic diversity in the newly formed daughter cells. In humans, crossing over produces genetically distinct haploid egg and sperm cells that undergo fertilization to produce unique offspring. Before cell division starts, the germ cell’s chromosome(s) undergo duplication in the S phase of the cell cycle. As the cells enter prophase I, duplicated...

