金属表面での直接電気化学的生物結合

Ariel L Furst¹, Matthew J Smith¹, Matthew B Francis^1,2

¹Department of Chemistry, University of California, Berkeley , Berkeley, California 94720-1460, United States.

Journal of the American Chemical Society

|September 1, 2017

まとめ

研究者たちは電気化学を用いて DNAを金面に結合させる新しい反応剤のない方法を開発しましたこの技術はDNA表面密度をより良く制御し,科学や医学におけるバイオセンサの応用を改善します

科学分野:

生物結合化学
表面科学
ナノテクノロジー

背景:

DNA の独特な識別能力と自己組み立て能力は,科学や医療機器に価値を持っています.
黄金の表面にDNAを結合させるためのチオール基を使用する現在の方法は制御不能であり,望ましくない基礎金属相互作用を引き起こす可能性があります.

研究の目的:

オリゴヌクレオチドを黄金の表面に結合させるための軽い,反応剤のない戦略を開発する.
制御可能なDNA表面密度を達成し,デバイスの性能を改善します.

主な方法:

アニリン改変DNAとカテキルコーティングの電極の電解活性化
カテコールからオキノンの酸化により,DNA結合が容易になる.
反応時間とカテコール含有量を変化させ DNAの表面覆いを制御する

主要な成果:

数分で高レベルのDNA結合を金の表面に達成した
反応パラメータを調整することで,最終的なDNA表面のカバーを正確に制御することが示されています.
ビスフェノールAを電気化学的に検出し,生細胞を捕獲する方法を成功裏に適用しました.

結論:

電気化学的に活性化された生物結合は,チオールベースのDNA結合に優れた代替手段を提供します.
この方法は,高度なデバイスプラットフォームのバイオ分子表面密度に対する強化された制御を提供します.
バイオセンシングと医療の応用におけるサイト固有のバイオ分子結合のための新しいアプローチを表しています

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