関連する実験動画

Updated: Feb 23, 2026

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
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CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

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タイプIIのCRISPR-Casが,Cas1-Cas2媒介のスペーサー統合によって免疫を確立する方法

Yibei Xiao1, Sherwin Ng1, Ki Hyun Nam2

  • 1Department of Molecular Biology and Genetics, Cornell University, 253 Biotechnology Building, Ithaca, New York 14853, USA.

Nature
|September 5, 2017
PubMed
まとめ

CRISPR-Casシステムは,Cas1-Cas2タンパク質を使用して,外来DNAスペーサーをCRISPR配列に統合し,適応免疫を形成します. この研究は,Enterococcus faecalisのスペーサー統合のステップ・バイ・ステップメカニズムを明らかにし,DNA曲線とリーダー・プロキシマル偏好を強調しています.

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