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関連する概念動画

Combinatorial Gene Control02:33

Combinatorial Gene Control

9.8K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

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Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
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Cooperative Binding of Transcription Regulators02:13

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The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

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The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
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The Eukaryotic Promoter Region02:40

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Co-activators and Co-repressors02:04

Co-activators and Co-repressors

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Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
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関連する実験動画

Updated: Feb 23, 2026

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions
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ポリコンブ型タンパク質は,PRC2複合体をCpG島と結びつける.

Haojie Li1, Robert Liefke2,3, Junyi Jiang1

  • 1Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, 19 Xinjiekouwai Avenue, Beijing 100875, China.

Nature
|September 5, 2017
PubMed
まとめ

ポリコンブ型 (PCL) タンパク質は,ポリコンブ抑制複合体2 (PRC2) をCpG島に誘導するために不可欠です. 翼状ヘリックス構造を含むこの結合機構は,転写の調節と細胞のアイデンティティの維持に不可欠です.

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科学分野:

  • エピジェネティクスと転写制御
  • クロマチン生物学
  • 遺伝子静止の分子メカニズム

背景:

  • ポリコンブ抑制複合体2 (PRC2) は遺伝子静止,細胞同一性,分化に不可欠である.
  • ポリコンブ型 (PCL) タンパク質 (例えば,PHF1,MTF2,PHF19) は,PRC2と結合し,その活性またはゲノム標的化を潜在的に調節する.
  • 低DNAメチル化を有するCpG島を含むCpGが豊富な地域ではPRC2結合部位が濃縮されているが,その徴募メカニズムは不明である.

研究 の 目的:

  • ポリコンブ型 (PCL) タンパク質がCpG豊富なDNAに結合する構造的基礎とメカニズムを解明する.
  • ポリコンブ抑制複合体2 (PRC2) をCpG島に誘導するPCLタンパク質の役割を決定する.
  • PCL-DNAの相互作用が,体内での転写調節に与える貢献を理解する.

主な方法:

  • CpG DNAとH3K36me3ペプチドに結合するPHF1とMTF2のN端ドメインの結晶構造の決定.
  • DNA結合特異性を特徴づけるための生化学的分析
  • マウスの胚性幹細胞におけるCpG島プロモーターへのPRC2徴募の評価

主要な成果:

  • PHF1とMTF2のN端領域は,非メチル化CpGモチーフを特定的に認識する翼状ヘリックス構造を採用する.
  • このDNA結合メカニズムは,正規の翼状ヘリックスモチーフと大きく異なる.
  • PCLタンパク質は,マウスの胚性幹細胞でPRC2をCpG島プロモーターに効率的に誘導するために不可欠です.

結論:

  • PCLタンパク質は,新しい翼状ヘリックス構造を介して,非メチル化CpGモチーフを直接結合する.
  • PCLタンパク質は,PRC2をCpG島に標的にする上で重要な役割を果たします.
  • この研究は,PCLタンパク質がPRC2のリクルートと転写調節における機能を直接的に証明している.