ミトスの転写とミトスの脱出中の遺伝子再活性化の波
Katherine C Palozola1,2, Greg Donahue2, Hong Liu3
1Institute for Regenerative Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-5157, USA.
Science (New York, N.Y.)
|September 16, 2017
まとめ
以前から考えられていたこととは対照的に ミトーシス中に多くの遺伝子が活性化し続けています この研究は,細胞がミトーシスを通して低レベルの転写を維持し,子細胞の成長のためにミトーシス出口の間にインターフェーズパターンを再確立することを明らかにしています.
科学分野:
- 分子生物学
- 細胞生物学
- 遺伝学
背景:
- ゲノムはミトーシス中に転写的に静かであると考えられています.
- 技術的な制約は,ミトーシスおよび出路中のトランスクリプションの敏感なマッピングを妨げています.
- インターフェーズ発現パターンを子細胞に変換するメカニズムは不明である.
研究 の 目的:
- ミトーシスとミトーシス脱出の間の遺伝子転写を調査する.
- 細胞循環の重要な段階で 転写された遺伝子を特定する
- インターフェーズ発現パターンが 子細胞にどのように伝達されるかを理解する.
主な方法:
- 5エチニルリジンのパルスラベリングは,ミトーシスと終了時にトランスクリプト.
- フルオレスセインイソチオシアネート-ウリジン5'-トリフォスファートのラベル
- RNA光 in situハイブリッド化について
- 定量逆転写ポリメラーゼ連鎖反応 (RT-qPCR)
主要な成果:
- 多数の遺伝子はミトーシスとミトーシス出路の間に転写を示します.
- 初期代写波は子細胞の成長と再構築のための遺伝子を活性化します.
- 細胞型特異的な遺伝子の活性化がミトーシス後に遅れている.
- 細胞の転写パターンの低いレベルはミトーシスを通して保持されます.
結論:
- ゲノムはミトーシス過程で完全には 書き換えられていません
- 転写パターンは主にミトーシスを通して低いレベルで維持されます.
- インターフェーズの転写幅はミトスの脱出時に回復し,子細胞の成長を準備する.
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