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CRISPR01:59

CRISPR

58.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.2K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.1K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.1K
Homologous Recombination02:31

Homologous Recombination

64.1K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
64.1K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

6.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.9K

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Updated: Feb 21, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

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CRISPR-Cas13によるRNAターゲティング

Omar O Abudayyeh1,2,3,4,5, Jonathan S Gootenberg1,2,3,4,6, Patrick Essletzbichler1,2,3,4

  • 1Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.

Nature
|October 5, 2017
PubMed
まとめ
この要約は機械生成です。

科学者はCRISPR-Cas13aを 哺乳類の細胞で精密なRNA操作のために設計しました この新しいツールは,既存の方法よりも高い特異性で,標的型RNAノックダウンと生細胞トランスクリプト追跡を提供します.

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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関連する実験動画

Last Updated: Feb 21, 2026

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

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In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

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科学分野:

  • 分子生物学
  • 遺伝学
  • バイオテクノロジー

背景:

  • RNAの操作と測定のための分子ツールが限られている.
  • RNA干渉のような現在の方法は 標的外効果があります
  • RNAを視覚化するには 外生的なタグが必要です

研究 の 目的:

  • CRISPR-Cas13aシステムを設計し,哺乳類の細胞でRNAをノックダウンし,結合させる.
  • RNAターゲティングにおけるLwaCas13aの有効性と特異性を評価する.
  • 生体細胞のRNAを研究するためのプログラム可能なプラットフォームを開発する.

主な方法:

  • 最も有効な変種 (LwaCas13a) を特定するために15人のCas13aオートロジーをスクリーニングした.
  • 哺乳類および植物細胞で異質的に発現するLwaCas13a
  • 触媒的に無活性なLwaCas13aをRNA結合と追跡に利用した.

主要な成果:

  • LwaCas13aは,レポーターと内生トランスクリプトの有効な標的的ノックダウンを示した.
  • RNAの干渉に匹敵するノックダウンレベルが達成されましたが,特異性が向上しました.
  • 非活性なLwaCas13aを用いた生細胞におけるトランスクリプトのプログラム可能な追跡が実証された.

結論:

  • CRISPR-Cas13aは,哺乳類の細胞におけるRNA研究のための汎用性のあるプラットフォームです.
  • LwaCas13aは,RNAのノックダウンと可視化のための特殊でプログラム可能なツールを提供します.
  • この技術はRNA関連疾患の治療開発に潜在的に応用できます