pituitary-specific 転写因子 GHF-1 は,ホメオボックスを含むタンパク質である
M Bodner1, J L Castrillo, L E Theill
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Cell
|November 4, 1988
まとめ
成長ホルモン因子1 (GHF-1) は,成長ホルモンの遺伝子発現に不可欠な,下垂体特異的転写因子である. 研究者らは,牛とネズミのGHF-1cDNAを分離し,細胞特異的な遺伝子調節におけるその役割を確認した.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- エンドクリノロジー エンドクリノロジー
背景:
- 成長ホルモン因子1 (GHF-1) は,下垂体特異の転写因子である.
- GHF-1は,成長ホルモン (GH) 遺伝子の細胞タイプ特異的発現に不可欠です.
研究 の 目的:
- 牛とネズミのGHF-1cDNAクローンを分離するために.
- GHF-1タンパク質とそのDNA結合領域を特徴づけるために.
主な方法:
- GHF-1cDNAクローンを牛とネズミの脳垂体図書館から分離した.
- GHF-1 cDNAのDNA配列解析について.
- バクテリアで発現するGHF-1融合タンパク質を用いたDNAase Iの足跡.
主要な成果:
- 牛とネズミのGHF-1cDNAを分離し,精製されたGHF-1と一致する33Kタンパク質をコーディングした.
- GHF-1配列には,ホームボックスのような領域が含まれています.
- DNAase Iの足跡は,ホームボックス領域がDNA結合ドメインであることを確認しました.
- GHF-1の発現は pituitary somatotropic lineage 細胞に限られている.
結論:
- GHF-1は,ホメオボックスDNA結合ドメインを持つ,下垂体特異的転写因子である.
- GHF-1の制限された発現は,選択的なGH遺伝子転写と相関しています.
- 哺乳類のホメオボックスタンパク質は,一般的に細胞型特異の遺伝子発現を制御する可能性がある.
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