免疫グロブリン遺伝子を活性化するヒトリンパ球特異の転写因子はホメオボックスタンパク質です
C Scheidereit1, J A Cromlish, T Gerster
1Laboratory of Biochemistry and Molecular Biology, Rockefeller University, New York, New York 10021.
Nature
|December 8, 1988
まとめ
リンパ性細胞に不可欠なヒトの転写因子OTF-2は,ドロソフィラ菌のタンパク質とDNA結合の類似性を共有しています. これは,これらの重要な規制要素とタンパク質の共通の進化史を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 進化生物学の進化生物学について
背景:
- 転写因子は遺伝子発現を調節する.
- ホメオドメインのタンパク質は,発達に関与する重要なDNA結合タンパク質です.
- OTF-2はヒトリンパ球特異の転写因子である.
研究 の 目的:
- ヒトの転写因子OTF-2のDNA結合特異性を調査する.
- OTF-2のDNA結合特性と無脊椎動物のホメオドメインタンパク質を比較する.
主な方法:
- OTF-2のホメオドメイン領域の分析.
- 標的DNA要素を決定するためのDNA結合測定法.
- 既知のドロソフィラホメオドメインのタンパク質結合部位との比較分析.
主要な成果:
- 人間のOTF-2タンパク質は,DNA結合に不可欠なホメオドメインを持っています.
- OTF-2はDNA要素に特異的に結合する.
- これらの元素は,ドロソフィラのホメオドメインタンパク質によっても認識されます.
結論:
- 哺乳類および無脊椎動物のホメオドメインタンパク質は,保存されたDNA結合特異性を示す.
- これは,ホメオドメインのタンパク質とそれらのDNA認識配列の種間の共進化を示唆しています.
- OTF-2は,保存された規制メカニズムを理解するためのモデルとして機能します.
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