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関連する概念動画

Mechanically-gated Ion Channels01:12

Mechanically-gated Ion Channels

7.9K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
7.9K
Ion Channels01:19

Ion Channels

91.7K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
91.7K
Voltage-gated Ion Channels01:26

Voltage-gated Ion Channels

11.2K
Voltage-gated ion channels are transmembrane proteins that open and close in response to changes in the membrane potential. They are present on the membranes of all electrically excitable cells such as neurons, heart, and muscle cells.
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
11.2K
Non-gated Ion Channels01:24

Non-gated Ion Channels

8.3K
Ion channels are specialized proteins on the plasma membrane that allow charged ions to pass down their electrochemical gradient. Their main function is to maintain the membrane potential which is critical for cell viability. These channels are either gated or non-gated and can transport more than a thousand ions within milliseconds for the cellular event to occur.
Compared to the gated ion channels, the non-gated channels, also known as leakage or passive channels, have no gating mechanism....
8.3K
Ligand-gated Ion Channels01:19

Ligand-gated Ion Channels

14.5K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
14.5K
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.3K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.3K

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関連する実験動画

Updated: Feb 20, 2026

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
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Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

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機械に敏感な合成イオンチャネル

Takahiro Muraoka1,2, Kaori Umetsu3, Kazuhito V Tabata4

  • 1School of Life Science and Technology, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8503, Japan.

Journal of the American Chemical Society
|October 28, 2017
PubMed
まとめ

研究者達は 機械的ストレスを感知する 細胞膜タンパク質を模倣する 合成分子を開発しました これらの緊張反応性アンフィフィルは 機械に敏感な新種の分子装置を 作り出す可能性を示しています

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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells
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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells

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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells

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関連する実験動画

Last Updated: Feb 20, 2026

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers
09:54

Multifunctional, Micropipette-based Method for Incorporation And Stimulation of Bacterial Mechanosensitive Ion Channels in Droplet Interface Bilayers

Published on: November 19, 2015

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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells
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Dextran Labeling and Uptake in Live and Functional Murine Cochlear Hair Cells

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Ex Vivo Analysis of Mechanically Activated Ca2+ Transients in Urothelial Cells
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科学分野:

  • バイオマテリアル科学
  • 分子工学
  • 超分子化学

背景:

  • 機械的ストレスは生物学的システムにとって重要な刺激であり,主に膜タンパク質によって感知されます.
  • 合成分子には機械力に対する感受性がほとんどなく,メカノ反応装置の開発を制限する.
  • 細胞膜のメカノ感受性イオンチャネルは,力検出のための生物学的青写真を提供します.

研究 の 目的:

  • テンション反応性トランスメブランアンフィフィルの設計と合成
  • これらの合成分子の 機械的反応とイオン輸送能力を調査する.
  • 合成システムの動作力を自然のメカノ感受性タンパク質と比較する.

主な方法:

  • 単体と3つのトランスメブランのアンフィフィルの発達.
  • リンパ膜にアンフィフィルの組み込み
  • 分子堆積と緊張への反応を評価するスペクトル分析.
  • 異なった機械的緊張条件下でのイオン輸送測定.

主要な成果:

  • 単一トランスメブランのアンフィフィルは,張力に依存する分子堆積の弱まりと強まりを示し,膨張する張力下でイオン輸送を誘発した.
  • 三重膜アンフィフィルは,張力のない状態でもイオン輸送のための単分子チャネルを形成し,張力が膨張すると活性が低下する.
  • これらの合成システムの動作力は,天然のメカノ敏感タンパク質と同等であることが判明した.

結論:

  • 合成トランスメブランアンフィフィルは 機械的ストレスに反応するように設計できます
  • 異なる分子構造 (単対三トランスメブラン) は,異なる張力反応機構とイオン輸送行動を示す.
  • この研究は,新しい合成メカノセンシティブ分子装置の開発に道を開きます.