空間的に異質な表面水の分散度 均衡状態の構造タンパク質表面
Ryan Barnes1, Sheng Sun1, Yann Fichou1
1Department of Chemistry and Biochemistry, University of California, Santa Barbara , Santa Barbara, California 93106, United States.
Journal of the American Chemical Society
|November 2, 2017
まとめ
水分化の水のダイナミクスはタンパク質の表面によって異なるが,水害性の領域では水分の拡散が遅い. この異質性は タンパク質が水分殻に情報をコードしていることを示しています
科学分野:
- バイオ物理学
- 物理化学
- 構造生物学
背景:
- 水分補給水は,タンパク質とリガンドの相互作用に不可欠です.
- 水の役割を理解するには タンパク質の表面の動態を調べる必要があります
- タンパク質の機能の鍵となるのは 水の性質の局所的な変化です
研究 の 目的:
- タンパク質の表面における特定部位の水分化ダイナミクスを調査する.
- 地元のタンパク質の表面特性と相関する.
- 異なる生物分子の表面における水の動態を比較する.
主な方法:
- オーバーハウザーのダイナミックな核極化 (ODNP) で,水のダイナミクスを探求する.
- タンパク質表面の水分化を決定する分子動力学 (MD) シミュレーション.
- 対象を特定した測定のための特定場所のラベル付け
主要な成果:
- 水分化水 (拡散水) のダイナミクスは,ケモタキシスY (CheY) タンパク質の表面全体に異質である.
- 水分拡散が遅いのは,タンパク質の表面部位が水害性の高いことと相関している.
- 球状タンパク質は,IDP,ペプチド,リポソームよりも異質な水力学を示す.
結論:
- タンパク質の表面構造が 局所的な水分動態を決定する.
- 構造化されたタンパク質は 補水殻の中に情報を 暗号化することができます
- 水分化水の異質性は,タンパク質の生体物理学の基本的な側面である.
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