関連する実験動画

Updated: Feb 18, 2026

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors
09:02

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

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DNA複製フォークで精密な編集により,ユカリオットの複合ゲノム工学が可能になる

Edward M Barbieri1, Paul Muir1, Benjamin O Akhuetie-Oni1

  • 1Department of Molecular, Cellular, & Developmental Biology, Yale University, New Haven, CT 06520, USA; Systems Biology Institute, Yale University, West Haven, CT 06516, USA.

Cell
|November 21, 2017
PubMed
まとめ

この研究は,酵母における新しいマルチプレックスゲノム工学の方法を紹介しています. 精密で効率的なDNA改変を 2本鎖の断絶なしに可能にし 経路工学のための広範な遺伝的多様性を生み出します

キーワード:
DNA複製ラッド51ゲノム編集同型再結合メタボリックエンジニアリングマルチプレックスゲノム工学自然産物ssDNA オリゴデオキシヌクレオチド

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