オートゴーナル筋繊維は,平面再生において異なる指導的役割を担っている
M Lucila Scimone1,2,3, Lauren E Cote1,2,3, Peter W Reddien1,2,3
1Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Nature
|November 24, 2017
まとめ
プラナリア筋繊維は再生において 明確な役割を果たします myoDやnkx1-1のような遺伝子によって制御される 特定の筋肉のタイプは 再生過程で新しい身体部位の形成とパターンが不可欠です
科学分野:
- 発達生物学
- 再生医療
- プラナリア生物学
背景:
- 再生は動物に共通しているが,異なる組織における位置情報の役割は不明である.
- 平面再生の研究では,しばしば筋肉で発現するパターニングに関与する位置制御遺伝子 (PCG) が特定されています.
- 平面筋の特異性と再生における異なる筋肉のサブセットの機能的な役割はよく理解されていません.
研究 の 目的:
- 平面再生における異なる筋肉繊維の異なる調節作用を調査する.
- 特定の筋肉のサブセットを特定するための遺伝的基礎を特定する.
- 筋肉の仕様が傷のシグナル伝達と 前後軸のパターンをどのように影響するかを理解する.
主な方法:
- RNA干渉 (RNAi) は,平面生物の遺伝子発現を阻害する.
- 遺伝子阻害による再生フェノタイプの特徴化
- myoDとnkx1-1を含む遺伝子発現パターンの分析
- 筋肉繊維の形成と方向の顕微鏡検査
主要な成果:
- myoD遺伝子は,再生の開始に不可欠な縦筋筋繊維の形成に不可欠です.
- 経路線繊維の喪失は 再生が完全に失敗する.
- nkx1-1遺伝子は,前後軸のパターンを影響する,円形の筋肉繊維形成に必要である.
- 円状の繊維の喪失は,軸の形成に欠陥をもたらし,例えば,軸が分岐し,頭が合体する.
結論:
- プラナリアの筋肉繊維の種類は,再生において異なる調節機能を備えている.
- myoDやnkx1-1のような遺伝子によって制御される 特定の筋肉のサブセットは 傷のシグナル伝達とパターン形成に 重要な役割を果たします
- 筋肉は単に収縮するだけでなく 再生過程を指揮する上で重要な役割を果たします
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